Pseudomonas putida S16, PPS_4485

Gene Feature Overview

Strain	Pseudomonas putida S16 GCF_000219705.1|latest
Locus Tag	PPS_4485
Name
Replicon	chromosome
Genomic location	5070125 - 5070730 (- strand)

Cross-References

RefSeq	YP_004703896.1
GI	339489368
Entrez	10940099
INSDC	AEJ15016.1
NCBI Locus Tag	PPS_4485
UniParc	UPI0002173DEE
UniProtKB Acc	F8G617
UniProtKB ID	F8G617_PSEP6
UniRef100	UniRef100_A0A099N6H2
UniRef50	UniRef50_P00436
UniRef90	UniRef90_P00436

Product

Feature Type	CDS
Coding Frame	1
Product Name	protocatechuate 3,4-dioxygenase subunit alpha
Synonyms
Evidence for Translation
Charge (pH 7)	-10.38
Kyte-Doolittle Hydrophobicity Value	-0.345
Molecular Weight (kDa)	22.3
Isoelectric Point (pI)	4.68

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3PCE	DIOXYGENASE	04/29/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-HYDROXYPHENYLACETATE	Pseudomonas putida	2.06	X-RAY DIFFRACTION	98.0
3PCF	DIOXYGENASE	06/27/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-FLURO-4-HYDROXYBENZOATE	Pseudomonas putida	2.15	X-RAY DIFFRACTION	98.0
3PCB	DIOXYGENASE	04/25/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-HYDROXYBENZOATE	Pseudomonas putida	2.19	X-RAY DIFFRACTION	98.0
3PCG	DIOXYGENASE	04/29/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH THE INHIBITOR 4-HYDROXYPHENYLACETATE	Pseudomonas putida	1.96	X-RAY DIFFRACTION	98.0
3PCD	DIOXYGENASE	11/24/97	PROTOCATECHUATE 3,4-DIOXYGENASE Y447H MUTANT	Pseudomonas putida	2.1	X-RAY DIFFRACTION	98.0
3PCH	DIOXYGENASE	07/01/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-CHLORO-4-HYDROXYBENZOATE	Pseudomonas putida	2.05	X-RAY DIFFRACTION	98.0
3PCI	DIOXYGENASE	07/02/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-IODO-4-HYDROXYBENZOATE	Pseudomonas putida	2.21	X-RAY DIFFRACTION	98.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
2-(3,4-Dihydroxyphenyl)Acetic Acid	DB01702	DrugBank	5.1.4	P00436	Protocatechuate 3,4-dioxygenase alpha chain	98.0	201	1.1e-114

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 102 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000152 (447 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PPS_4485 Search term: protocatechuate 3,4-dioxygenase subunit alpha

Human Homologs

References