Pseudomonas putida BIRD-1, PPUBIRD1_3225

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas putida BIRD-1
GCF_000183645.1|latest
Locus Tag
PPUBIRD1_3225
Name
Replicon chromosome
Genomic location 3609619 - 3610707 (+ strand)

Cross-References

RefSeq YP_005931029.1
GI 386012752
EC Number 3.5.1.38
Entrez 12521394
NCBI Locus Tag PPUBIRD1_3225

Product

Feature Type CDS
Coding Frame 1
Product Name
Glutaminase-asparaginase
Synonyms
Evidence for Translation
Charge (pH 7) 1.21
Kyte-Doolittle Hydrophobicity Value -0.108
Molecular Weight (kDa) 38.6
Isoelectric Point (pI) 7.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6WYW HYDROLASE 05/13/20 Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase in complex with L-Asp at pH 4.5 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 2.13 X-RAY DIFFRACTION 99.7
6WYY HYDROLASE 05/13/20 Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase in complex with L-Glu at pH 6.5 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 1.35 X-RAY DIFFRACTION 99.7
6WZ4 HYDROLASE 05/13/20 Complex of mutant (K173M) of Pseudomonas 7A Glutaminase-Asparaginase with L-Asp at pH 6. Covalent acyl-enzyme intermediate Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 1.58 X-RAY DIFFRACTION 99.4
6WZ8 HYDROLASE 05/13/20 Complex of Pseudomonas 7A Glutaminase-Asparaginase with Citrate Anion at pH 5.5. Covalent Acyl-Enzyme Intermediate Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 1.7 X-RAY DIFFRACTION 99.7
6WYX HYDROLASE 05/13/20 Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase in complex with L-Asp at pH 5.0 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 1.48 X-RAY DIFFRACTION 99.7
6WZ6 HYDROLASE 05/13/20 Complex of mutant (K173M) of Pseudomonas 7A Glutaminase-Asparaginase with L-Glu at pH 5. Covalent acyl-enzyme intermediate Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 1.15 X-RAY DIFFRACTION 99.4
6WYZ HYDROLASE 05/13/20 Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase (mutant K173M) in complex with D-Glu at pH 5.5 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 1.7 X-RAY DIFFRACTION 99.4

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Threonine-Aspartic Ester DB01817 DrugBank 5.1.4 P00805 L-asparaginase 2 45.4 359 2.6e-74
2-Amino-6-Oxo-Hexanoic Acid DB02571 DrugBank 5.1.4 Q88K39 Glutaminase-asparaginase 99.7 362 1.4e-195
4-Carboxy-4-Aminobutanal DB04388 DrugBank 5.1.4 Q88K39 Glutaminase-asparaginase 99.7 362 1.4e-195

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 351 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001273 (453 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PPUBIRD1_3225

Human Homologs

References

No references are associated with this feature.