Pseudomonas aeruginosa UCBPP-PA14, PA14_06750 (nirS)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_06750
Name	nirS
Replicon	chromosome
Genomic location	588371 - 590077 (- strand)
Transposon Mutants	2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	13 transposon mutants in orthologs

Cross-References

RefSeq	YP_788689.1
GI	116054245
Entrez	4383885
INSDC	ABJ15481.1
NCBI Locus Tag	PA14_06750
UniParc	UPI000045D429
UniProtKB Acc	Q02TP7
UniProtKB ID	Q02TP7_PSEAB
UniRef100	UniRef100_Q02TP7
UniRef50	UniRef50_P24474
UniRef90	UniRef90_P24474

Product

Feature Type	CDS
Coding Frame	1
Product Name	nitrite reductase
Synonyms
Evidence for Translation
Charge (pH 7)	5.04
Kyte-Doolittle Hydrophobicity Value	-0.395
Molecular Weight (kDa)	62.7
Isoelectric Point (pI)	8.21

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
1BL9	OXIDOREDUCTASE	07/20/98	CONFORMATIONAL CHANGES OCCURRING UPON REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.9	X-RAY DIFFRACTION	99.6
1NIR	NITRITE REDUCTASE	06/17/97	OXYDIZED NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.15	X-RAY DIFFRACTION	99.6
1HZU	OXIDOREDUCTASE	01/26/01	DOMAIN SWING UPON HIS TO ALA MUTATION IN NITRITE REDUCTASE OF PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.7	X-RAY DIFFRACTION	99.4
1N90	OXIDOREDUCTASE	09/04/98	FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.9	X-RAY DIFFRACTION	99.6
1GJQ	OXIDOREDUCTASE	08/01/01	Pseudomonas aeruginosa cd1 nitrite reductase reduced cyanide complex	PSEUDOMONAS AERUGINOSA	2.7	X-RAY DIFFRACTION	99.6
5GUW	MEMBRANE PROTEIN	08/31/16	Complex of Cytochrome cd1 Nitrite Reductase and Nitric Oxide Reductase in Denitrification of Pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	3.2	X-RAY DIFFRACTION	99.6
1HZV	OXIDOREDUCTASE	01/26/01	DOMAIN SWING UPON HIS TO ALA MUTATION IN NITRITE REDUCTASE OF PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.83	X-RAY DIFFRACTION	99.4
1NNO	OXIDOREDUCTASE	07/20/98	CONFORMATIONAL CHANGES OCCURRING UPON NO BINDING IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.65	X-RAY DIFFRACTION	99.6
1N15	OXIDOREDUCTASE	09/04/98	FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.9	X-RAY DIFFRACTION	99.6
1N50	OXIDOREDUCTASE	09/04/98	FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA	Pseudomonas aeruginosa	2.9	X-RAY DIFFRACTION	99.6
6TSI	OXIDOREDUCTASE	12/20/19	cd1 nitrite reductase NirS with bound dihydro-heme d1	Pseudomonas aeruginosa PAO1	2.38	X-RAY DIFFRACTION	99.6
6TPO	OXIDOREDUCTASE	12/13/19	Conformation of cd1 nitrite reductase NirS without bound heme d1	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.861	X-RAY DIFFRACTION	99.6

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 48 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000503 (354 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_06750 Search term: nirS Search term: nitrite reductase

Human Homologs

References