Pseudomonas aeruginosa UCBPP-PA14, PA14_07500 (rmcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
GCF_000014625.1|latest
Locus Tag
PA14_07500
Name
rmcA
Replicon chromosome
Genomic location 643347 - 647084 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 9 transposon mutants in orthologs

Cross-References

RefSeq YP_788748.1
GI 116054303
Entrez 4384222
INSDC ABJ15539.1
NCBI Locus Tag PA14_07500
UniParc UPI000045D43F
UniProtKB Acc Q02TI8
UniProtKB ID Q02TI8_PSEAB
UniRef100 UniRef100_Q02TI8
UniRef50 UniRef50_Q4K4W0
UniRef90 UniRef90_Q9I5W1

Product

Feature Type CDS
Coding Frame 1
Product Name
redox regulator of c_di_GMP, RmcA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -29.53
Kyte-Doolittle Hydrophobicity Value -0.176
Molecular Weight (kDa) 139.7
Isoelectric Point (pI) 5.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5M3C HYDROLASE 10/14/16 Structure of the hybrid domain (GGDEF-EAL) of PA0575 from Pseudomonas aeruginosa PAO1 at 2.8 Ang. with GTP and Ca2+ bound to the active site of the GGDEF domain PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 563 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000558 (508 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_07500
Search term: rmcA

References

Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa.
Mantoni F, Paiardini A, Brunotti P, D'Angelo C, Cervoni L, Paone A, Cappellacci L, Petrelli R, Ricciutelli M, Leoni L, Rampioni G, Arcovito A, Rinaldo S, Cutruzzolà F, Giardina G.
FEBS J. 2018 Oct;285(20):3815-3834
PubMed ID: 30106221
Electron-shuttling antibiotics structure bacterial communities by modulating cellular levels of c-di-GMP.
Okegbe C, Fields BL, Cole SJ, Beierschmitt C, Morgan CJ, Price-Whelan A, Stewart RC, Lee VT, Dietrich LEP
Proc Natl Acad Sci U S A 2017 Jun 27;114(26):E5236-E5245
PubMed ID: 28607054