Pseudomonas aeruginosa UCBPP-PA14, PA14_33820 (pvdQ)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_33820
Name	pvdQ
Replicon	chromosome
Genomic location	3004988 - 3007276 (+ strand)
Transposon Mutants	2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	3 transposon mutants in orthologs

Cross-References

RefSeq	YP_790857.1
GI	116050326
Entrez	4380447
INSDC	ABJ11562.1
NCBI Locus Tag	PA14_33820
UniParc	UPI000045D2DE
UniProtKB Acc	Q02MH9
UniProtKB ID	Q02MH9_PSEAB
UniRef100	UniRef100_Q02MH9
UniRef50	UniRef50_Q9I194
UniRef90	UniRef90_Q9I194

Product

Feature Type	CDS
Coding Frame	1
Product Name	penicillin acylase-related protein
Synonyms
Evidence for Translation
Charge (pH 7)	0.50
Kyte-Doolittle Hydrophobicity Value	-0.348
Molecular Weight (kDa)	84.1
Isoelectric Point (pI)	7.10

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4WKV	HYDROLASE/HYDROLASE Inhibitor	10/03/14	n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ	Pseudomonas aeruginosa	2.1434	X-RAY DIFFRACTION	100.0
2WYC	HYDROLASE	11/16/09	The quorum quenching N-acyl homoserine lactone acylase PvdQ in complex with 3-oxo-lauric acid	PSEUDOMONAS AERUGINOSA	1.9	X-RAY DIFFRACTION	100.0
2WYE	HYDROLASE	11/16/09	The quorum quenching N-acyl homoserine lactone acylase PvdQ is an Ntn- Hydrolase with an unusual substrate-binding pocket	PSEUDOMONAS AERUGINOSA	1.8	X-RAY DIFFRACTION	100.0
4WKT	HYDROLASE/HYDROLASE Inhibitor	10/03/14	n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ	Pseudomonas aeruginosa	1.782	X-RAY DIFFRACTION	100.0
4WKS	HYDROLASE/HYDROLASE INHIBITOR	10/03/14	n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ	Pseudomonas aeruginosa	1.629	X-RAY DIFFRACTION	100.0
4K2F	HYDROLASE/HYDROLASE INHIBITOR	04/09/13	Structure of Pseudomonas aeruginosa PvdQ bound to BRD-A08522488	Pseudomonas aeruginosa	1.99	X-RAY DIFFRACTION	100.0
4WKU	HYDROLASE/HYDROLASE Inhibitor	10/03/14	n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	100.0
4BTH	HYDROLASE	06/18/13	The LeuA146Trp,PheB24Tyr Double Mutant of the Quorum Quenching N-acyl Homoserine Lactone Acylase PvdQ Has an Altered Substrate Specificity Towards Small Acyl Chains	PSEUDOMONAS AERUGINOSA	1.9	X-RAY DIFFRACTION	99.8
3L91	HYDROLASE	01/04/10	Structure of Pseudomonas aerugionsa PvdQ bound to octanoate	Pseudomonas aeruginosa	1.66	X-RAY DIFFRACTION	100.0
2WYB	HYDROLASE	11/16/09	The quorum quenching N-acyl homoserine lactone acylase PvdQ with a covalently bound dodecanoic acid	PSEUDOMONAS AERUGINOSA	2.1	X-RAY DIFFRACTION	100.0
4K2G	HYDROLASE/HYDROLASE INHIBITOR	04/09/13	Structure of Pseudomonas aeruginosa PvdQ bound to BRD-A33442372	Pseudomonas aeruginosa	2.3	X-RAY DIFFRACTION	100.0
3SRC	HYDROLASE/HYDROLASE INHIBITOR	07/07/11	Structure of Pseudomonas aeruginosa PvdQ bound to NS2028	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	100.0
3SRB	HYDROLASE/HYDROLASE INHIBITOR	07/07/11	Structure of Pseudomonas aeruginosa PvdQ bound to SMER28	Pseudomonas aeruginosa	1.8	X-RAY DIFFRACTION	100.0
3SRA	HYDROLASE	07/07/11	Structure of Pseudomonas aerugionsa PvdQ covalently acylated with myristic acid from PVDIq	Pseudomonas aeruginosa PAO1	2.3	X-RAY DIFFRACTION	100.0
5UBK	HYDROLASE	12/20/16	Inactive S1A/N269D-cpPvdQ mutant in complex with the pyoverdine precursor PVDIq reveals a specific binding pocket for the D-Tyr of this substrate	Pseudomonas aeruginosa	2.55	X-RAY DIFFRACTION	99.6
5UBL	HYDROLASE	12/20/16	A circularly permuted version of PvdQ (cpPvdQ)	Pseudomonas aeruginosa	1.8	X-RAY DIFFRACTION	100.0
4M1J	HYDROLASE	08/02/13	Crystal structure of Pseudomonas aeruginosa PvdQ in complex with a transition state analogue	Pseudomonas aeruginosa	1.8	X-RAY DIFFRACTION	100.0
2WYD	HYDROLASE	11/16/09	The quorum quenching N-acyl homoserine lactone acylase PvdQ in complex with dodecanoic acid	PSEUDOMONAS AERUGINOSA	1.901	X-RAY DIFFRACTION	100.0
3L94	HYDROLASE	01/04/10	Structure of PvdQ covalently acylated with myristate	Pseudomonas aeruginosa	1.95	X-RAY DIFFRACTION	100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
Lauric acid	DB03017	DrugBank	5.1.4	Q9I194	Acyl-homoserine lactone acylase PvdQ	99.9	762	0.0e+00

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 181 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003142 (466 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_33820 Search term: pvdQ Search term: penicillin acylase-related protein

Human Homologs

References