Pseudomonas aeruginosa UCBPP-PA14, PA14_53940 (prpB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_53940
Name
prpB
Replicon chromosome
Genomic location 4782285 - 4783181 (+ strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq YP_792486.1
GI 116048714
EC Number 4.1.3.30
Entrez 4381663
INSDC ABJ09950.1
NCBI Locus Tag PA14_53940
UniParc UPI00000C5159
UniProtKB Acc Q02HV0
UniProtKB ID Q02HV0_PSEAB
UniRef100 UniRef100_W0YNY4
UniRef50 UniRef50_Q56062
UniRef90 UniRef90_W0YNY4

Product

Feature Type CDS
Coding Frame 1
Product Name
2-methylisocitrate lyase
Synonyms
Evidence for Translation
Charge (pH 7) -5.92
Kyte-Doolittle Hydrophobicity Value 0.032
Molecular Weight (kDa) 32.1
Isoelectric Point (pI) 5.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6T5M LYASE 10/16/19 Crystal structure of 2-methylisocitrate lyase (PrpB) from Pseudomonas aeruginosa in complex with Mg(II)-pyruvate. Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.76 X-RAY DIFFRACTION 100.0
6T4V LYASE 10/15/19 Crystal structure of 2-methylisocitrate lyase (PrpB) from Pseudomonas aeruginosa in apo form. Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.807 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Caenorhabditis elegans
Notes
Infection model C. elegans slow killing assay.
Reference 22911607

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 342 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000769 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_53940
Search term: prpB

Human Homologs

References

No references are associated with this feature.