Pseudomonas aeruginosa M18, PAM18_0426 (mexB)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa M18
GCF_000226155.1|latest |
| Locus Tag |
PAM18_0426
|
| Name |
mexB
|
| Replicon | chromosome |
| Genomic location | 466754 - 469855 (+ strand) |
Cross-References
| RefSeq | YP_005973017.1 |
| GI | 386056495 |
| Entrez | 12564444 |
| NCBI Locus Tag | PAM18_0426 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexB
|
| Synonyms | |
| Evidence for Translation | |
| Charge (pH 7) | -10.89 |
| Kyte-Doolittle Hydrophobicity Value | 0.269 |
| Molecular Weight (kDa) | 111181.8 |
| Isoelectric Point (pI) | 5.30 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AMR gene predictions from CARD database
Identified using the Resistance Gene Identifier (RGI)
| Model Name | Definition | Accession | Model Type (?) | SNP | Cutoff | Bit Score | Percent Identity | CARD Version |
| MexB | MexB is the inner membrane multidrug exporter of the efflux complex MexAB-OprM. [PMID:19361527, PMID:21178960, PMID:14973037, PMID:20583998] | ARO:3000378 | protein homolog model | Strict | 1953.3 | 99.61 | 3.2.5 |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 6IIA | MEMBRANE PROTEIN | 10/04/18 | MexB in complex with LMNG | Pseudomonas aeruginosa PAO1 | 2.91 | X-RAY DIFFRACTION | 99.7 |
| 6IOL | MEMBRANE PROTEIN | 10/30/18 | Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state) | Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1 | 3.76 | ELECTRON MICROSCOPY | 99.7 |
| 3W9I | MEMBRANE PROTEIN | 04/04/13 | Structural basis for the inhibition of bacterial multidrug exporters | Pseudomonas aeruginosa | 2.71 | X-RAY DIFFRACTION | 99.7 |
| 3W9J | MEMBRANE PROTEIN | 04/04/13 | Structural basis for the inhibition of bacterial multidrug exporters | Pseudomonas aeruginosa | 3.15 | X-RAY DIFFRACTION | 99.7 |
| 2V50 | MEMBRANE PROTEIN | 10/01/08 | The Missing Part of the Bacterial MexAB-OprM System: Structural determination of the Multidrug Exporter MexB | PSEUDOMONAS AERUGINOSA PA01 | 3 | X-RAY DIFFRACTION | 99.7 |
| 6IOK | MEMBRANE PROTEIN | 10/30/18 | Cryo-EM structure of multidrug efflux pump MexAB-OprM (0 degree state) | Pseudomonas aeruginosa PAO1 | 3.64 | ELECTRON MICROSCOPY | 99.7 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000417 (2093 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PAM18_0426
Search term: mexB
|
Human Homologs
References
No references are associated with this feature.