Pseudomonas aeruginosa M18, PAM18_3883 (toxA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas aeruginosa M18
GCF_000226155.1|latest
Locus Tag
PAM18_3883
Name
toxA
Replicon chromosome
Genomic location 4315653 - 4317569 (+ strand)

Cross-References

RefSeq YP_005976466.1
GI 386059944
Entrez 12567937
NCBI Locus Tag PAM18_3883

Product

Feature Type CDS
Coding Frame 1
Product Name
exotoxin A
Synonyms
Evidence for Translation
Charge (pH 7) -16.15
Kyte-Doolittle Hydrophobicity Value -0.267
Molecular Weight (kDa) 69.3
Isoelectric Point (pI) 5.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1ZM3 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of the apo eEF2-ETA complex Pseudomonas aeruginosa; Saccharomyces cerevisiae 3.07 X-RAY DIFFRACTION 99.5
1ZM4 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of the eEF2-ETA-bTAD complex Pseudomonas aeruginosa; Saccharomyces cerevisiae 2.9 X-RAY DIFFRACTION 99.5
6EDG TRANSFERASE 08/09/18 Pseudomonas exotoxin A domain III T18H477L Pseudomonas aeruginosa 1.47 X-RAY DIFFRACTION 97.2
1DMA ADP-RIBOSYLATION 04/28/95 DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH NICOTINAMIDE AND AMP Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 99.5
1IKQ TRANSFERASE 05/04/01 Pseudomonas Aeruginosa Exotoxin A, wild type Pseudomonas aeruginosa 1.62 X-RAY DIFFRACTION 99.5
1XK9 TRANSFERASE 09/28/04 Pseudomanas exotoxin A in complex with the PJ34 inhibitor Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 99.5
1AER ADP-RIBOSYLATION 12/11/95 DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH BETA-TAD Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 99.5
2ZIT BIOSYNTHETIC PROTEIN/TRANSFERASE 02/24/08 Structure of the eEF2-ExoA-NAD+ complex Pseudomonas aeruginosa; Saccharomyces cerevisiae 3 X-RAY DIFFRACTION 99.5
1IKP TRANSFERASE 05/04/01 Pseudomonas Aeruginosa Exotoxin A, P201Q, W281A mutant Pseudomonas aeruginosa 1.45 X-RAY DIFFRACTION 99.2
1ZM9 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of eEF2-ETA in complex with PJ34 Pseudomonas aeruginosa; Saccharomyces cerevisiae 2.8 X-RAY DIFFRACTION 99.5
1ZM2 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA Pseudomonas aeruginosa; Saccharomyces cerevisiae 3.07 X-RAY DIFFRACTION 99.5

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE DB08348 DrugBank 5.1.4 Q5EK40 Cholix toxin 32.6 693 6.8e-84

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001108 (295 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PAM18_3883
Search term: toxA
Search term: exotoxin A

Human Homologs

References

No references are associated with this feature.