Pseudomonas aeruginosa PAO1, PA0082 (tssA1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0082
Name
tssA1
Replicon chromosome
Genomic location 100124 - 101158 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248772.1
GI 15595280
Affymetrix PA0082_at
DNASU PaCD00006035
Entrez 879402
GenBank AAG03472.1
INSDC AAG03472.1
NCBI Locus Tag PA0082
protein_id(GenBank) gb|AAG03472.1|AE004447_5|gnl|PseudoCAP|PA0082
TIGR NTL03PA00083
UniParc UPI00000C4F23
UniProtKB Acc Q9I750
UniProtKB ID Q9I750_PSEAE
UniRef100 UniRef100_Q9I750
UniRef50 UniRef50_Q4K3N9
UniRef90 UniRef90_Q9I750

Product

Feature Type CDS
Coding Frame 1
Product Name
TssA1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -23.53
Kyte-Doolittle Hydrophobicity Value 0.010
Molecular Weight (kDa) 36.6
Isoelectric Point (pI) 4.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4228
Category Not available / Not known
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 12023080
Source 2
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 81 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000081 (1178 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0082
Search term: tssA1
Search term: TssA1

Human Homologs

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading) 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
TssA forms a gp6-like ring attached to the type VI secretion sheath.
Planamente S, Salih O, Manoli E, Albesa-Jové D, Freemont PS, Filloux A
EMBO J 2016 Aug 1;35(15):1613-27
PubMed ID: 27288401