Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0091
|
Name |
vgrG1
Synonym: vgrG1a |
Replicon | chromosome |
Genomic location | 110976 - 112907 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 10 transposon mutants in orthologs |
RefSeq | NP_248781.1 |
GI | 15595289 |
Affymetrix | PA0091_at |
Entrez | 879511 |
GenBank | AAG03481.1 |
INSDC | AAG03481.1 |
NCBI Locus Tag | PA0091 |
protein_id(GenBank) | gb|AAG03481.1|AE004447_14|gnl|PseudoCAP|PA0091 |
TIGR | NTL03PA00092 |
UniParc | UPI00000C4F2C |
UniProtKB Acc | Q9I741 |
UniProtKB ID | Q9I741_PSEAE |
UniRef100 | UniRef100_Q9I741 |
UniRef50 | UniRef50_Q9I741 |
UniRef90 | UniRef90_Q9I741 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
VgrG1
|
Synonyms |
VgrG1a |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -6.10 |
Kyte-Doolittle Hydrophobicity Value | -0.542 |
Molecular Weight (kDa) | 72.0 |
Isoelectric Point (pI) | 6.37 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6H3L | TOXIN | 07/19/18 | Structure of VgrG1 in the Type VI secretion pre-firing VgrG1-Tse6-EagT6-EF-Tu-Tsi6 complex | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 4.2 | ELECTRON MICROSCOPY | 100.0 |
4UHV | STRUCTURAL PROTEIN | 03/25/15 | The structure of VgrG1, the needle tip of the bacterial Type VI Secretion System | PSEUDOMONAS AERUGINOSA | 2 | X-RAY DIFFRACTION | 100.0 |
4MTK | TOXIN | 09/19/13 | Crystal structure of PA0091 VgrG1, the central spike of the Type VI Secretion System | Pseudomonas aeruginosa | 3.322 | X-RAY DIFFRACTION | 100.0 |
6H3N | TOXIN | 07/19/18 | Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs | Pseudomonas aeruginosa PAO1 | 3.25 | ELECTRON MICROSCOPY | 100.0 |
Source 1 | |
Database | VFDB : VF0334 |
Category | Hcp secretion island-1 encoded type VI secretion system (H-T6SS) |
Evidence |
ECO:0000305
curator inference used in manual assertion
|
Host Organism | |
Notes | |
Reference | 16763151 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 156 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000090 (3342 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0091
Search term: vgrG1
Search term: VgrG1
|
Rhs elements comprise three subfamilies which diverged prior to acquisition by Escherichia coli.
Wang YD, Zhao S, Hill CW
J Bacteriol 1998 Aug;180(16):4102-10
PubMed ID: 9696756
|
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun 9;312(5779):1526-30
PubMed ID: 16763151
|
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
|
Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins.
Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jové D, Filloux A
J Biol Chem 2011 Apr 8;286(14):12317-27
PubMed ID: 21325275
|
An interbacterial NAD(P)(+) glycohydrolase toxin requires elongation factor Tu for delivery to target cells.
Whitney JC, Quentin D, Sawai S, LeRoux M, Harding BN, Ledvina HE, Tran BQ, Robinson H, Goo YA, Goodlett DR, Raunser S, Mougous JD
Cell 2015 Oct 22;163(3):607-19
PubMed ID: 26456113
|