Pseudomonas aeruginosa PAO1, PA0172 (siaA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 194757 - 196748 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentSiaA is a functional Mg2+/Mn2+-dependent protein-phosphatase (PP2C-type) with activity towards the phosphorylated threonine T68 of SiaC (PA0170) [PMID:33156827]
CommentInvolved in postranscriptional regulation of CupA fimbriae via transcriptional activation of rsmZ
CommentThe SiaA-PP2C domain is arranged as a homodimer in the crystal asymmetric unit (PDB-ID: 6K4E) and its phosphatase activity can be inhibited by fumonisin B1[PMID:33156827]
CommentThe SiaABC threonine phosphorylation pathway controls aggregate/biofilm formation in response to carbon availability (glucose, succinate, ethanol, 2,3-butanediol)[PMID:33156827]
CommentInvolved in surfactant-induced (SDS) aggregation.


RefSeq NP_248862.1
GI 15595370
Affymetrix PA0172_at
Entrez 879545
GenBank AAG03562.1
INSDC AAG03562.1
NCBI Locus Tag PA0172
protein_id(GenBank) gb|AAG03562.1|AE004455_3|gnl|PseudoCAP|PA0172
UniParc UPI00000C4F7B
UniProtKB Acc Q9I6W0
UniRef100 UniRef100_Q9I6W0
UniRef50 UniRef50_Q9I6W0
UniRef90 UniRef90_Q9I6W0


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.03
Kyte-Doolittle Hydrophobicity Value -0.206
Molecular Weight (kDa) 74.0
Isoelectric Point (pI) 7.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6K4E SIGNALING PROTEIN 05/23/19 SiaA-PP2C domain of Pseudomonas aeruginosa Pseudomonas aeruginosa 2.094 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 243 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000170 (323 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0172
Search term: siaA
Search term: SiaA

Human Homologs


Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy.
Klebensberger J, Lautenschlager K, Bressler D, Wingender J, Philipp B
Environ Microbiol 2007 Sep;9(9):2247-59
PubMed ID: 17686022
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Klebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B
Environ Microbiol 2009 Dec;11(12):3073-86
PubMed ID: 19638175
SiaA/D Interconnects c-di-GMP and RsmA Signaling to Coordinate Cellular Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions.
Colley B, Dederer V, Carnell M, Kjelleberg S, Rice SA, Klebensberger J
Front Microbiol 2016;7:179
PubMed ID: 26955366
The SiaABC threonine phosphorylation pathway controls biofilm formation in response to carbon availability in Pseudomonas aeruginosa.
Poh WH, Lin J, Colley B, Müller N, Goh BC, Schleheck D, El Sahili A, Marquardt A, Liang Y, Kjelleberg S, Lescar J, Rice SA, Klebensberger J
PLoS One 2020;15(11):e0241019
PubMed ID: 33156827