Pseudomonas aeruginosa PAO1, PA0295

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 331762 - 332823 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
CommentStructural similarity to 1A99 (putrescine-binding protein from Escherichia coli, root-mean-square deviation: 0.6 angstroms). Sequence similarity to 1A99 (99%/37,54%) (PUT). Final predicted ligand: putrescine (PMID: 31631799)


RefSeq NP_248986.1
GI 15595492
Affymetrix PA0295_at
Entrez 880681
GenBank AAG03684.1
INSDC AAG03684.1
NCBI Locus Tag PA0295
protein_id(GenBank) gb|AAG03684.1|AE004467_6|gnl|PseudoCAP|PA0295
UniParc UPI00000C4FEA
UniProtKB Acc Q9I6J6
UniRef100 UniRef100_A0A0A8RBP2
UniRef50 UniRef50_A4XP47
UniRef90 UniRef90_W0YNY5


Feature Type CDS
Coding Frame 1
Product Name
probable periplasmic polyamine binding protein
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.13
Kyte-Doolittle Hydrophobicity Value -0.214
Molecular Weight (kDa) 39.0
Isoelectric Point (pI) 5.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 306 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000292 (324 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0295

Human Homologs


A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T
DNA Res 1996 Jun 30;3(3):137-55
PubMed ID: 8905232
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799