Pseudomonas aeruginosa PAO1, PA0300 (spuD)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: potF2
Replicon chromosome
Genomic location 338437 - 339540 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_248991.1
GI 15595497
Affymetrix PA0300_potF2_at
Entrez 880655
GenBank AAG03689.1
INSDC AAG03689.1
NCBI Locus Tag PA0300
protein_id(GenBank) gb|AAG03689.1|AE004468_1|gnl|PseudoCAP|PA0300
UniParc UPI00000C4FEF
UniProtKB Acc Q9I6J1
UniRef100 UniRef100_Q02UB7
UniRef50 UniRef50_P31133
UniRef90 UniRef90_Q02UB7


Feature Type CDS
Coding Frame 1
Product Name
polyamine transport protein
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 1.14
Kyte-Doolittle Hydrophobicity Value -0.292
Molecular Weight (kDa) 40.6
Isoelectric Point (pI) 7.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3TTK TRANSPORT PROTEIN 09/14/11 Crystal structure of apo-SpuD Pseudomonas aeruginosa 2.97 X-RAY DIFFRACTION 99.7
3TTM TRANSPORT PROTEIN 09/15/11 Crystal structure of SpuD in complex with putrescine Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 306 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000297 (535 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0300
Search term: spuD

Human Homologs


Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome.
Pistocchi R, Kashiwagi K, Miyamoto S, Nukui E, Sadakata Y, Kobayashi H, Igarashi K
J Biol Chem 1993 Jan 5;268(1):146-52
PubMed ID: 8416922
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994