Pseudomonas aeruginosa PAO1, PA0604 (agtB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0604
Name
agtB
Replicon chromosome
Genomic location 665948 - 666994 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentStructural similarity to 4I1D (ABC transporter substrate-binding protein from Bradyrhizobium japonicum, root-mean-square deviation: 0.8 angstroms). Sequence similarity to 4EUO (99%/36,22%) (ABU). Final predicted ligand: gamma-aminobutyric acid (PMID: 31631799)

Cross-References

RefSeq NP_249295.1
GI 15595801
Affymetrix PA0604_at
DNASU PaCD00006265
Entrez 879023
GenBank AAG03993.1
INSDC AAG03993.1
NCBI Locus Tag PA0604
protein_id(GenBank) gb|AAG03993.1|AE004496_9|gnl|PseudoCAP|PA0604
TIGR NTL03PA00605
UniParc UPI00000C50D4
UniProtKB Acc Q9I5T4
UniProtKB ID Q9I5T4_PSEAE
UniRef100 UniRef100_S0IV94
UniRef50 UniRef50_I3UHC8
UniRef90 UniRef90_W0YM22

Product

Feature Type CDS
Coding Frame 1
Product Name
AgtB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -1.09
Kyte-Doolittle Hydrophobicity Value -0.277
Molecular Weight (kDa) 38.2
Isoelectric Point (pI) 5.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 170 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000587 (511 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0604
Search term: agtB
Search term: AgtB

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Functional characterization of the agtABCD and agtSR operons for 4-aminobutyrate and 5-aminovalerate uptake and regulation in Pseudomonas aeruginosa PAO1.
Chou HT, Li JY, Lu CD
Curr Microbiol 2014 Jan;68(1):59-63
PubMed ID: 23982201
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799