Pseudomonas aeruginosa PAO1, PA0913 (mgtE)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 996038 - 997486 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249604.1
GI 15596110
Affymetrix PA0913_mgtE_at
Entrez 878306
GenBank AAG04302.1
INSDC AAG04302.1
NCBI Locus Tag PA0913
protein_id(GenBank) gb|AAG04302.1|AE004525_10|gnl|PseudoCAP|PA0913
UniParc UPI00000C51B4
UniProtKB Acc Q9I544
UniRef100 UniRef100_W0YKI5
UniRef50 UniRef50_I7KFQ9
UniRef90 UniRef90_W0YKI5


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Detected using LC-MS/MS (53.82 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -30.83
Kyte-Doolittle Hydrophobicity Value 0.036
Molecular Weight (kDa) 53.8
Isoelectric Point (pI) 4.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 484 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000879 (554 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0913
Search term: mgtE
Search term: MgtE


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning of the mgtE Mg2+ transporter from Providencia stuartii and the distribution of mgtE in gram-negative and gram-positive bacteria.
Townsend DE, Esenwine AJ, George J, Bross D, Maguire ME, Smith RL
J. Bacteriol. 1995 Sep;177(18):5350-4
PubMed ID: 7665526
Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.
Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E, Nakamura Y, Miyajima N, Hirosawa M, Sugiura M, Sasamoto S, Kimura T, Hosouchi T, Matsuno A, Muraki A, Nakazaki N, Naruo K, Okumura S, Shimpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S
DNA Res. 1996 Jun;3(3):109-36
PubMed ID: 8905231
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
MgtE is a dual-function protein in Pseudomonas aeruginosa.
Coffey BM, Akhand SS, Anderson GG
Microbiology (Reading, Engl.) 2014 Jun;160(Pt 6):1200-13
PubMed ID: 24722909
The Pseudomonas aeruginosa magnesium transporter MgtE inhibits transcription of the type III secretion system.
Anderson GG, Yahr TL, Lovewell RR, O'Toole GA
Infect. Immun. 2010 Mar;78(3):1239-49
PubMed ID: 20028803