Pseudomonas aeruginosa PAO1, PA0931 (pirA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0931
Name
pirA
Synonym: pirA
Replicon chromosome
Genomic location 1018230 - 1020458 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 13 transposon mutants in orthologs
Commentsecond ferric enterobactin receptor gene next to pfeA.

Cross-References

RefSeq NP_249622.1
GI 15596128
Affymetrix PA0931_at
Entrez 881093
GenBank AAG04320.1
INSDC AAG04320.1
NCBI Locus Tag PA0931
protein_id(GenBank) gb|AAG04320.1|AE004527_7|gnl|PseudoCAP|PA0931
TIGR NTL03PA00932
UniParc UPI00000C51C4
UniProtKB Acc Q9I527
UniProtKB ID Q9I527_PSEAE
UniRef100 UniRef100_Q9I527
UniRef50 UniRef50_P05825
UniRef90 UniRef90_Q9I527

Product

Feature Type CDS
Coding Frame 1
Product Name
ferric enterobactin receptor PirA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -9.34
Kyte-Doolittle Hydrophobicity Value -0.550
Molecular Weight (kDa) 80.9
Isoelectric Point (pI) 5.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5FP2 METAL TRANSPORT 11/27/15 Crystal structure of the siderophore receptor PirA from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.97 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 340 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000897 (3061 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0931
Search term: pirA

Human Homologs

References

The Pseudomonas aeruginosa pirA gene encodes a second receptor for ferrienterobactin and synthetic catecholate analogues.
Ghysels B, Ochsner U, Möllman U, Heinisch L, Vasil M, Cornelis P, Matthijs S
FEMS Microbiol Lett 2005 May 15;246(2):167-74
PubMed ID: 15899402
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular analysis of the Escherichia coli ferric enterobactin receptor FepA.
Armstrong SK, Francis CL, McIntosh MA
J Biol Chem 1990 Aug 25;265(24):14536-43
PubMed ID: 2201687
Cloning and characterization of the ferric enterobactin receptor gene (pfeA) of Pseudomonas aeruginosa.
Dean CR, Poole K
J Bacteriol 1993 Jan;175(2):317-24
PubMed ID: 8419284
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
Ochsner UA, Vasil ML
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):4409-14
PubMed ID: 8633080