Pseudomonas aeruginosa PAO1, PA0410 (pilI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0410
Name
pilI
Replicon chromosome
Genomic location 450509 - 451045 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249101.1
GI 15595607
Affymetrix PA0410_pilI_at
Entrez 878186
GenBank AAG03799.1
INSDC AAG03799.1
NCBI Locus Tag PA0410
protein_id(GenBank) gb|AAG03799.1|AE004478_4|gnl|PseudoCAP|PA0410
TIGR NTL03PA00411
UniParc UPI0000131ACB
UniProtKB Acc P43502
UniProtKB ID PILI_PSEAE
UniRef100 UniRef100_P43502
UniRef50 UniRef50_P43502
UniRef90 UniRef90_P43502

Product

Feature Type CDS
Coding Frame 1
Product Name
twitching motility protein PilI
Product Name Confidence: Class 1
Synonyms type 4 fimbrial biogenesis protein PilI
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.39
Kyte-Doolittle Hydrophobicity Value 0.112
Molecular Weight (kDa) 19.9
Isoelectric Point (pI) 6.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili twitching motility related proteins
Evidence
Host Organism
Notes
Reference 9224879
Source 2
Database Victors : 4216
Category Type IV pili twitching motility related proteins
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 212 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000401 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0410
Search term: pilI

Human Homologs

References

Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus.
Darzins A
Mol Microbiol 1994 Jan;11(1):137-53
PubMed ID: 7908398
The molecular genetics of type-4 fimbriae in Pseudomonas aeruginosa--a review.
Mattick JS, Whitchurch CB, Alm RA
Gene 1996 Nov 7;179(1):147-55
PubMed ID: 8955641