Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0426
|
Name |
mexB
|
Replicon | chromosome |
Genomic location | 473191 - 476331 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 8 transposon mutants in orthologs |
RefSeq | NP_249117.1 |
GI | 15595623 |
Affymetrix | PA0426_mexB_at |
Entrez | 877852 |
GenBank | AAG03815.1 |
INSDC | AAD45628.1 |
NCBI Locus Tag | PA0426 |
protein_id(GenBank) | gb|AAG03815.1|AE004479_10|gnl|PseudoCAP|PA0426 |
TIGR | NTL03PA00427 |
UniParc | UPI000012F022 |
UniProtKB Acc | P52002 |
UniProtKB ID | MEXB_PSEAE |
UniRef100 | UniRef100_P52002 |
UniRef50 | UniRef50_P52002 |
UniRef90 | UniRef90_P52002 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexB
|
Synonyms |
RND multidrug efflux transporter MexB |
Evidence for Translation |
Detected using LC-MS/MS (112.79 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -9.89 |
Kyte-Doolittle Hydrophobicity Value | 0.274 |
Molecular Weight (kDa) | 112.8 |
Isoelectric Point (pI) | 5.40 |
Individual Mappings | |
Additional evidence for subcellular localization |
Identified using the Resistance Gene Identifier (RGI)
Model Name | Definition | Accession | Model Type (?) | SNP | Cutoff | Bit Score | Percent Identity | CARD Version |
MexB | MexB is the inner membrane multidrug exporter of the efflux complex MexAB-OprM. [PMID:19361527, PMID:21178960, PMID:14973037, PMID:20583998] | ARO:3000378 | protein homolog model | Strict | 1984.5 | 99.9 | 3.2.5 |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2V50 | MEMBRANE PROTEIN | 10/01/08 | The Missing Part of the Bacterial MexAB-OprM System: Structural determination of the Multidrug Exporter MexB | PSEUDOMONAS AERUGINOSA PA01 | 3 | X-RAY DIFFRACTION | 100.0 |
6IOK | MEMBRANE PROTEIN | 10/30/18 | Cryo-EM structure of multidrug efflux pump MexAB-OprM (0 degree state) | Pseudomonas aeruginosa PAO1 | 3.64 | ELECTRON MICROSCOPY | 100.0 |
6IOL | MEMBRANE PROTEIN | 10/30/18 | Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state) | Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1 | 3.76 | ELECTRON MICROSCOPY | 100.0 |
3W9I | MEMBRANE PROTEIN | 04/04/13 | Structural basis for the inhibition of bacterial multidrug exporters | Pseudomonas aeruginosa | 2.71 | X-RAY DIFFRACTION | 100.0 |
6IIA | MEMBRANE PROTEIN | 10/04/18 | MexB in complex with LMNG | Pseudomonas aeruginosa PAO1 | 2.91 | X-RAY DIFFRACTION | 100.0 |
3W9J | MEMBRANE PROTEIN | 04/04/13 | Structural basis for the inhibition of bacterial multidrug exporters | Pseudomonas aeruginosa | 3.15 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000417 (2093 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0426
Search term: mexB
|
Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine.
Poole K, Heinrichs DE, Neshat S
Mol Microbiol 1993 Nov;10(3):529-44
PubMed ID: 7968531
|
Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.
Li XZ, Nikaido H, Poole K
Antimicrob Agents Chemother 1995 Sep;39(9):1948-53
PubMed ID: 8540696
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|