Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0509
|
Name |
nirN
|
Replicon | chromosome |
Genomic location | 571111 - 572592 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_249200.1 |
GI | 15595706 |
Affymetrix | PA0509_nirN_at |
Entrez | 878428 |
GenBank | AAG03898.1 |
INSDC | AAG03898.1 |
NCBI Locus Tag | PA0509 |
protein_id(GenBank) | gb|AAG03898.1|AE004488_2|gnl|PseudoCAP|PA0509 |
TIGR | NTL03PA00510 |
UniParc | UPI00000C5098 |
UniProtKB Acc | Q9I609 |
UniProtKB ID | Q9I609_PSEAE |
UniRef100 | UniRef100_W0YNA8 |
UniRef50 | UniRef50_F8GTF5 |
UniRef90 | UniRef90_A6UYX0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
NirN
|
Synonyms | |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 2.79 |
Kyte-Doolittle Hydrophobicity Value | -0.203 |
Molecular Weight (kDa) | 54.0 |
Isoelectric Point (pI) | 7.54 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6RTE | OXIDOREDUCTASE | 05/23/19 | Dihydro-heme d1 dehydrogenase NirN in complex with DHE | Pseudomonas aeruginosa | 1.94 | X-RAY DIFFRACTION | 99.8 |
6RTD | OXIDOREDUCTASE | 05/23/19 | Dihydro-heme d1 dehydrogenase NirN in complex with DHE | Pseudomonas aeruginosa | 2.36 | X-RAY DIFFRACTION | 99.8 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000494 (709 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0509
Search term: nirN
Search term: NirN
|
Two c-type cytochromes, NirM and NirC, encoded in the nir gene cluster of Pseudomonas aeruginosa act as electron donors for nitrite reductase.
Hasegawa N, Arai H, Igarashi Y
Biochem Biophys Res Commun 2001 Nov 16;288(5):1223-30
PubMed ID: 11700043
|
Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis.
Kawasaki S, Arai H, Kodama T, Igarashi Y
J Bacteriol 1997 Jan;179(1):235-42
PubMed ID: 8982003
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
NirN protein from Pseudomonas aeruginosa is a novel electron-bifurcating dehydrogenase catalyzing the last step of heme d1 biosynthesis.
Adamczack J, Hoffmann M, Papke U, Haufschildt K, Nicke T, Bröring M, Sezer M, Weimar R, Kuhlmann U, Hildebrandt P, Layer G
J Biol Chem 2014 Oct 31;289(44):30753-30762
PubMed ID: 25204657
|