Pseudomonas aeruginosa PAO1, PA0518 (nirM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0518
Name
nirM
Replicon chromosome
Genomic location 578029 - 578343 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249209.1
GI 15595715
Affymetrix PA0518_nirM_at
DNASU PaCD00006218
Entrez 882220
GenBank AAG03907.1
INSDC AAG03907.1
NCBI Locus Tag PA0518
protein_id(GenBank) gb|AAG03907.1|AE004488_11|gnl|PseudoCAP|PA0518
TIGR NTL03PA00519
UniParc UPI0000126C4A
UniProtKB Acc P00099
UniProtKB ID CY551_PSEAE
UniRef100 UniRef100_P00099
UniRef50 UniRef50_P95339
UniRef90 UniRef90_P00099

Product

Feature Type CDS
Coding Frame 1
Product Name
cytochrome c-551 precursor
Product Name Confidence: Class 1
Synonyms cytochrome c8
c551
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.16
Kyte-Doolittle Hydrophobicity Value -0.056
Molecular Weight (kDa) 11.0
Isoelectric Point (pI) 7.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
351C ELECTRON TRANSPORT 07/20/81 STRUCTURE OF CYTOCHROME C551 FROM P. AERUGINOSA REFINED AT 1.6 ANGSTROMS RESOLUTION AND COMPARISON OF THE TWO REDOX FORMS Pseudomonas aeruginosa 1.6 X-RAY DIFFRACTION 100.0
2EXV ELECTRON TRANSPORT 11/09/05 Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.86 X-RAY DIFFRACTION 98.8
1DVV ELECTRON TRANSPORT 01/22/00 SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA Pseudomonas aeruginosa NOT SOLUTION NMR 93.9
3X39 ELECTRON TRANSPORT 01/16/15 Domain-swapped dimer of Pseudomonas aeruginosa cytochrome c551 Pseudomonas aeruginosa PAO1 1.5 X-RAY DIFFRACTION 100.0
451C ELECTRON TRANSPORT 07/20/81 STRUCTURE OF CYTOCHROME C551 FROM P. AERUGINOSA REFINED AT 1.6 ANGSTROMS RESOLUTION AND COMPARISON OF THE TWO REDOX FORMS Pseudomonas aeruginosa 1.6 X-RAY DIFFRACTION 100.0
2PAC ELECTRON TRANSPORT 05/05/93 SOLUTION STRUCTURE OF FE(II) CYTOCHROME C551 FROM PSEUDOMONAS AERUGINOSA AS DETERMINED BY TWO-DIMENSIONAL 1H NMR Pseudomonas aeruginosa NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 88 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000502 (369 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0518
Search term: nirM

Human Homologs

References

The structural gene for cytochrome c551 from Pseudomonas aeruginosa. The nucleotide sequence shows a location downstream of the nitrite reductase gene.
Nordling M, Young S, Karlsson BG, Lundberg LG
FEBS Lett 1990 Jan 1;259(2):230-2
PubMed ID: 2152881
Investigation of the structure of oxidized Pseudomonas aeruginosa cytochrome c-551 by NMR: comparison of observed paramagnetic shifts and calculated pseudocontact shifts.
Timkovich R, Cai M
Biochemistry 1993 Nov 2;32(43):11516-23
PubMed ID: 8218218