Pseudomonas aeruginosa PAO1, PA0524 (norB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0524
Name
norB
Replicon chromosome
Genomic location 582455 - 583855 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249215.1
GI 15595721
Affymetrix PA0524_norB_at
Entrez 882193
GenBank AAG03913.1
INSDC AAG03913.1
NCBI Locus Tag PA0524
protein_id(GenBank) gb|AAG03913.1|AE004489_5|gnl|PseudoCAP|PA0524
TIGR NTL03PA00525
UniParc UPI0000130371
UniProtKB Acc Q59647
UniProtKB ID NORB_PSEAE
UniRef100 UniRef100_Q59647
UniRef50 UniRef50_P98008
UniRef90 UniRef90_Q59647

Product

Feature Type CDS
Coding Frame 1
Product Name
nitric-oxide reductase subunit B
Product Name Confidence: Class 1
Synonyms cytochrome B subunit of nitric oxide reductase
Evidence for Translation
Charge (pH 7) 7.88
Kyte-Doolittle Hydrophobicity Value 0.781
Molecular Weight (kDa) 52.3
Isoelectric Point (pI) 9.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5GUW MEMBRANE PROTEIN 08/31/16 Complex of Cytochrome cd1 Nitrite Reductase and Nitric Oxide Reductase in Denitrification of Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 3.2 X-RAY DIFFRACTION 99.8
5GUX MEMBRANE PROTEIN 08/31/16 Cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with xenon Mus musculus; Pseudomonas aeruginosa PAO1 3.3 X-RAY DIFFRACTION 99.8
3WFE IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced and cyanide-bound cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.49 X-RAY DIFFRACTION 99.8
3O0R IMMUNE SYSTEM/OXIDOREDUCTASE 07/20/10 Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 99.8
3WFB IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 99.8
3WFD IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced and acetaldoxime-bound cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 99.8
3WFC IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced and carbonmonoxide-bound cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 211 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000508 (374 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0524
Search term: norB

Human Homologs

References

Purification and characterization of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC
J Biol Chem 1991 Feb 5;266(4):2203-9
PubMed ID: 1899237
Extended x-ray absorption fine structure and electron paramagnetic resonance of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC, Kolodziej AF, Orme-Johnson WH, Bunker G
J Biol Chem 1991 Feb 5;266(4):2210-8
PubMed ID: 1846618
The structural genes for nitric oxide reductase from Pseudomonas aeruginosa.
Arai H, Igarashi Y, Kodama T
Biochim Biophys Acta 1995 Apr 4;1261(2):279-84
PubMed ID: 7711073