Pseudomonas aeruginosa PAO1, PA1177 (napE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1177
Name
napE
Replicon chromosome
Genomic location 1276617 - 1276784 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249868.1
GI 15596374
Affymetrix PA1177_napE_at
DNASU PaCD00006542
Entrez 878584
GenBank AAG04566.1
INSDC AAG04566.1
NCBI Locus Tag PA1177
protein_id(GenBank) gb|AAG04566.1|AE004547_12|gnl|PseudoCAP|PA1177
TIGR NTL03PA01178
UniParc UPI00000C528F
UniProtKB Acc Q9I4G0
UniProtKB ID Q9I4G0_PSEAE
UniRef100 UniRef100_W0YMA5
UniRef50 UniRef50_A6V921
UniRef90 UniRef90_A6V921

Product

Feature Type CDS
Coding Frame 1
Product Name
periplasmic nitrate reductase protein NapE
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) -0.08
Kyte-Doolittle Hydrophobicity Value 0.705
Molecular Weight (kDa) 6.0
Isoelectric Point (pI) 6.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 33 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001135 (343 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1177
Search term: napE

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Isolation of periplasmic nitrate reductase genes from Rhodobacter sphaeroides DSM 158: structural and functional differences among prokaryotic nitrate reductases.
Reyes F, Roldán MD, Klipp W, Castillo F, Moreno-Vivián C
Mol Microbiol 1996 Mar;19(6):1307-18
PubMed ID: 8730872
Periplasmic nitrate-reducing system of the phototrophic bacterium Rhodobacter sphaeroides DSM 158: transcriptional and mutational analysis of the napKEFDABC gene cluster.
Reyes F, Gavira M, Castillo F, Moreno-Vivián C
Biochem J 1998 May 1;331 ( Pt 3)(Pt 3):897-904
PubMed ID: 9560320