Pseudomonas aeruginosa PAO1, PA1247 (aprE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1247
Name
aprE
Replicon chromosome
Genomic location 1352525 - 1353823 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249938.1
GI 15596444
Affymetrix PA1247_aprE_at
Entrez 881276
GenBank AAG04636.1
INSDC AAG04636.1
NCBI Locus Tag PA1247
protein_id(GenBank) gb|AAG04636.1|AE004554_4|gnl|PseudoCAP|PA1247
TIGR NTL03PA01248
UniParc UPI0000125CB6
UniProtKB Acc Q03025
UniProtKB ID APRE_PSEAE
UniRef100 UniRef100_Q03025
UniRef50 UniRef50_Q03025
UniRef90 UniRef90_Q03025

Product

Feature Type CDS
Coding Frame 1
Product Name
alkaline protease secretion protein AprE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 2.70
Kyte-Doolittle Hydrophobicity Value -0.328
Molecular Weight (kDa) 48.1
Isoelectric Point (pI) 8.69

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 246 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001199 (788 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1247
Search term: aprE

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa alkaline protease: evidence for secretion genes and study of secretion mechanism.
Guzzo J, Pages JM, Duong F, Lazdunski A, Murgier M
J Bacteriol 1991 Sep;173(17):5290-7
PubMed ID: 1832151
Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
Duong F, Lazdunski A, Cami B, Murgier M
Gene 1992 Nov 2;121(1):47-54
PubMed ID: 1427098
Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
Duong F, Lazdunski A, Cami B, Murgier M
Gene 1992 Nov 2;121(1):47-54
PubMed ID: 1427098