Pseudomonas aeruginosa UCBPP-PA14, PA14_05530 (mexA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_05530
Name	mexA
Replicon	chromosome
Genomic location	486775 - 487926 (+ strand)
Transposon Mutants	1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	YP_788599.1
GI	116054156
Entrez	4384293
INSDC	ABJ15392.1
NCBI Locus Tag	PA14_05530
UniParc	UPI000012F021
UniProtKB Acc	Q02TY7
UniProtKB ID	Q02TY7_PSEAB
UniRef100	UniRef100_P52477
UniRef50	UniRef50_P52477
UniRef90	UniRef90_P52477

Product

Feature Type	CDS
Coding Frame	1
Product Name	RND multidrug efflux membrane fusion protein MexA
Synonyms
Evidence for Translation
Charge (pH 7)	3.22
Kyte-Doolittle Hydrophobicity Value	-0.229
Molecular Weight (kDa)	41.0
Isoelectric Point (pI)	9.15

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name	Definition	Accession	Model Type (?)	SNP	Cutoff	Bit Score	Percent Identity	CARD Version
MexA	MexA is the membrane fusion protein of the MexAB-OprM multidrug efflux complex. [PMID:15117957]	ARO:3000377	protein homolog model		Perfect	716.1	100.0	3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6TA5	ANTIMICROBIAL PROTEIN	10/29/19	OprM-MexA complex from the MexAB-OprM Pseudomonas aeruginosa whole assembly reconstituted in nanodiscs	Pseudomonas aeruginosa	3.2	ELECTRON MICROSCOPY	100.0
1T5E	TRANSPORT PROTEIN	05/04/04	The structure of MexA	Pseudomonas aeruginosa	3	X-RAY DIFFRACTION	100.0
6TA6	ANTIMICROBIAL PROTEIN	10/29/19	MexAB assembly of the Pseudomonas MexAB-OprM efflux pump reconstituted in nanodiscs	Pseudomonas aeruginosa	3.2	ELECTRON MICROSCOPY	100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
Glycerin	DB09462	DrugBank	5.1.4	P52477	Multidrug resistance protein MexA	100.0	383	2.9e-204

Virulence Evidence

Source 1
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Caenorhabditis elegans
Notes
Infection model	C. elegans fast killing model
Reference	9989496
Source 2
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Arabidopsis thaliana
Notes
Infection model	Arabidopsis leaf infiltration model
Reference	9989496

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 394 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000416 (526 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_05530 Search term: mexA Search term: RND multidrug efflux membrane fusion protein MexA

Human Homologs

References