Pseudomonas aeruginosa PAO1, PA4001 (sltB1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4001
Name
sltB1
Synonym: mltB2
Replicon chromosome
Genomic location 4481230 - 4482252 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252690.1
GI 15599196
Affymetrix PA4001_mltB2_at
Entrez 878946
GenBank AAG07388.1
INSDC AAG07388.1
NCBI Locus Tag PA4001
protein_id(GenBank) gb|AAG07388.1|AE004817_12|gnl|PseudoCAP|PA4001
TIGR NTL03PA04001
UniParc UPI00000C5BB9
UniProtKB Acc Q9HX24
UniProtKB ID Q9HX24_PSEAE
UniRef100 UniRef100_Q9HX24
UniRef50 UniRef50_Q9HX24
UniRef90 UniRef90_Q9HX24

Product

Feature Type CDS
Coding Frame 1
Product Name
soluble lytic transglycosylase B
Product Name Confidence: Class 1
Synonyms membrane-bound lytic transglycosylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.79
Kyte-Doolittle Hydrophobicity Value -0.239
Molecular Weight (kDa) 37.8
Isoelectric Point (pI) 5.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ANR LYASE 03/22/12 Crystal structure of soluble lytic Transglycosylase SltB1 from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.84 X-RAY DIFFRACTION 100.0
5O8X LYASE 06/14/17 The X-ray Structure of Catenated Lytic Transglycosylase SltB1 from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 229 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001682 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4001
Search term: sltB1

Human Homologs

References

Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.
Blackburn NT, Clarke AJ
Biochemistry 2002 Jan 22;41(3):1001-13
PubMed ID: 11790124
Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli.
Ehlert K, Höltje JV, Templin MF
Mol Microbiol 1995 May;16(4):761-8
PubMed ID: 7476170