Pseudomonas aeruginosa PAO1, PA4025 (eutC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4025
Name
eutC
Synonym: eutC
Replicon chromosome
Genomic location 4506913 - 4507734 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPart of the PA4022-eat-eutBC operon responsible for ethanolamine catabolism.

Cross-References

RefSeq NP_252714.1
GI 15599220
Affymetrix PA4025_r_at
DNASU PaCD00007757
Entrez 879003
GenBank AAG07412.1
INSDC AAG07412.1
NCBI Locus Tag PA4025
protein_id(GenBank) gb|AAG07412.1|AE004819_5|gnl|PseudoCAP|PA4025
TIGR NTL03PA04025
UniParc UPI000012A286
UniProtKB Acc Q9HX02
UniProtKB ID EUTC_PSEAE
UniRef100 UniRef100_B7V7T3
UniRef50 UniRef50_Q4K5E1
UniRef90 UniRef90_B7V7T3

Product

Feature Type CDS
Coding Frame 1
Product Name
ethanolamine-ammonia lyase, small subunit, EutC
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.89
Kyte-Doolittle Hydrophobicity Value -0.240
Molecular Weight (kDa) 30.1
Isoelectric Point (pI) 6.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 137 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001658 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4025
Search term: eutC

Human Homologs

References

Cloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium.
Faust LR, Connor JA, Roof DM, Hoch JA, Babior BM
J Biol Chem 1990 Jul 25;265(21):12462-6
PubMed ID: 2197274
Ethanolamine Catabolism in Pseudomonas aeruginosa PAO1 Is Regulated by the Enhancer-Binding Protein EatR (PA4021) and the Alternative Sigma Factor RpoN.
Lundgren BR, Sarwar Z, Pinto A, Ganley JG, Nomura CT
J Bacteriol 2016 Sep 1;198(17):2318-29
PubMed ID: 27325678