Pseudomonas aeruginosa PAO1, PA4109 (ampR)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4109
Name	ampR
Replicon	chromosome
Genomic location	4592990 - 4593880 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_252798.1
GI	15599304
Affymetrix	PA4109_ampR_at
Entrez	877983
GenBank	AAG07496.1
INSDC	CAA38523.2
NCBI Locus Tag	PA4109
protein_id(GenBank)	gb|AAG07496.1|AE004827_5|gnl|PseudoCAP|PA4109
TIGR	NTL03PA04109
UniParc	UPI0000125A26
UniProtKB Acc	P24734
UniProtKB ID	AMPR_PSEAE
UniRef100	UniRef100_P24734
UniRef50	UniRef50_P24734
UniRef90	UniRef90_P24734

Product

Feature Type	CDS
Coding Frame	1
Product Name	transcriptional regulator AmpR Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	1.53
Kyte-Doolittle Hydrophobicity Value	0.127
Molecular Weight (kDa)	32.6
Isoelectric Point (pI)	7.36

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5MMH	TRANSCRIPTION	12/09/16	The X-Ray Structure of the Effector Domain of the Transcriptional Regulator AmpR of Pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	2.2	X-RAY DIFFRACTION	99.5

Virulence Evidence

Source 1
Database	PseudoCAP
Category	AmpR
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Caenorhabditis elegans
Notes
Infection model	C. Elegans fast killing (paralytic) assay
Reference	22479525

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 456 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001142 (1134 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4109 Search term: ampR Search term: transcriptional regulator AmpR

Human Homologs

References

Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase. Lodge JM, Minchin SD, Piddock LJ, Busby SJ Biochem J 1990 Dec 15;272(3):627-31 PubMed ID: 2125210

Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter. Lodge J, Busby S, Piddock L FEMS Microbiol Lett 1993 Aug 1;111(2-3):315-20 PubMed ID: 8405939

The regulatory repertoire of Pseudomonas aeruginosa AmpC ß-lactamase regulator AmpR includes virulence genes. Balasubramanian D, Schneper L, Merighi M, Smith R, Narasimhan G, Lory S, Mathee K PLoS One 2012;7(3):e34067 PubMed ID: 22479525

Structural and functional characterization of Pseudomonas aeruginosa global regulator AmpR. Caille O, Zincke D, Merighi M, Balasubramanian D, Kumari H, Kong KF, Silva-Herzog E, Narasimhan G, Schneper L, Lory S, Mathee K J Bacteriol 2014 Nov;196(22):3890-902 PubMed ID: 25182487

Pseudomonas aeruginosa AmpR: an acute-chronic switch regulator. Balasubramanian D, Kumari H, Mathee K Pathog Dis 2015 Mar;73(2):1-14 PubMed ID: 25066236

Role of Pseudomonas aeruginosa AmpR on β-lactam and non-β-lactam transient cross-resistance upon pre-exposure to subinhibitory concentrations of antibiotics. Kumari H, Balasubramanian D, Zincke D, Mathee K J. Med. Microbiol. 2014 Apr;63(Pt 4):544-55 PubMed ID: 24464693

LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames. Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K J Proteomics 2014 Jan 16;96:328-342 PubMed ID: 24291602

Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat shock and oxidative stress response. Balasubramanian D, Kumari H, Jaric M, Fernandez M, Turner KH, Dove SL, Narasimhan G, Lory S, Mathee K Nucleic Acids Res 2014 Jan;42(2):979-98 PubMed ID: 24157832

A dynamic and intricate regulatory network determines Pseudomonas aeruginosa virulence. Balasubramanian D, Schneper L, Kumari H, Mathee K Nucleic Acids Res 2013 Jan 7;41(1):1-20 PubMed ID: 23143271