Pseudomonas aeruginosa PAO1, PA0075 (pppA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: tagG1
Replicon chromosome
Genomic location 90106 - 90834 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_248765.1
GI 15595273
Affymetrix PA0075_at
DNASU PaCD00006033
Entrez 879341
GenBank AAG03465.1
INSDC AAG03465.1
NCBI Locus Tag PA0075
protein_id(GenBank) gb|AAG03465.1|AE004446_13|gnl|PseudoCAP|PA0075
UniParc UPI00000C4F1C
UniProtKB Acc Q9I757
UniRef100 UniRef100_Q9I757
UniRef50 UniRef50_Q9I757
UniRef90 UniRef90_Q9I757


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Synonyms TagG1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.90
Kyte-Doolittle Hydrophobicity Value 0.021
Molecular Weight (kDa) 25.9
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JKV HYDROLASE 03/02/19 PppA, a key regulatory component of T6SS in Pseudomonas aeruginosa Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Reference 16763151

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 392 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000074 (811 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0075
Search term: pppA
Search term: PppA

Human Homologs


Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa.
Mougous JD, Gifford CA, Ramsdell TL, Mekalanos JJ
Nat Cell Biol 2007 Jul;9(7):797-803
PubMed ID: 17558395
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading) 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433