Pseudomonas aeruginosa PAO1, PA0082 (tssA1)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0082
|
| Name |
tssA1
|
| Replicon | chromosome |
| Genomic location | 100124 - 101158 (+ strand) |
| Transposon Mutants | 3 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_248772.1 |
| GI | 15595280 |
| Affymetrix | PA0082_at |
| DNASU | PaCD00006035 |
| Entrez | 879402 |
| GenBank | AAG03472.1 |
| INSDC | AAG03472.1 |
| NCBI Locus Tag | PA0082 |
| protein_id(GenBank) | gb|AAG03472.1|AE004447_5|gnl|PseudoCAP|PA0082 |
| TIGR | NTL03PA00083 |
| UniParc | UPI00000C4F23 |
| UniProtKB Acc | Q9I750 |
| UniProtKB ID | Q9I750_PSEAE |
| UniRef100 | UniRef100_Q9I750 |
| UniRef50 | UniRef50_Q4K3N9 |
| UniRef90 | UniRef90_Q9I750 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
TssA1
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -23.53 |
| Kyte-Doolittle Hydrophobicity Value | 0.010 |
| Molecular Weight (kDa) | 36.6 |
| Isoelectric Point (pI) | 4.14 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0334 |
| Category | Hcp secretion island-1 encoded type VI secretion system (H-T6SS) |
| Evidence |
ECO:0000305
curator inference used in manual assertion
|
| Host Organism | |
| Notes | |
| Reference | 16763151 |
| Source 2 | |
| Database | Victors : 4228 |
| Category | Not available / Not known |
| Evidence |
ECO:0000015
mutant phenotype evidence
|
| Host Organism | Rattus norvegicus |
| Notes | |
| Infection model | Rat agar bead model of chronic lung infection |
| Reference | 12023080 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 81 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000081 (1212 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0082
Search term: tssA1
Search term: TssA1
|
Human Homologs
References
|
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
|
|
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433
|
|
TssA forms a gp6-like ring attached to the type VI secretion sheath.
Planamente S, Salih O, Manoli E, Albesa-Jové D, Freemont PS, Filloux A
EMBO J. 2016 Aug;35(15):1613-27
PubMed ID: 27288401
|