Pseudomonas aeruginosa PAO1, PA0083 (tssB1)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 101247 - 101765 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_248773.1
GI 15595281
Affymetrix PA0083_at
DNASU PaCD00006036
Entrez 879476
GenBank AAG03473.1
INSDC AAG03473.1
NCBI Locus Tag PA0083
protein_id(GenBank) gb|AAG03473.1|AE004447_6|gnl|PseudoCAP|PA0083
UniParc UPI00000C4F24
UniProtKB Acc Q9I749
UniRef100 UniRef100_W0YKW6
UniRef50 UniRef50_J2YIR2
UniRef90 UniRef90_J2YIR2


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 2
Evidence for Translation
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:4.7, molecular weight:16.8, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Charge (pH 7) -3.00
Kyte-Doolittle Hydrophobicity Value -0.251
Molecular Weight (kDa) 18.8
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5N8N STRUCTURAL PROTEIN 02/23/17 Contracted sheath of a Pseudomonas aeruginosa type six secretion system consisting of TssB1 and TssC1 Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
4UQZ PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1 1.599 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Reference 16763151

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 107 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000082 (1224 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0083
Search term: tssB1
Search term: TssB1

Human Homologs


A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading) 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802