Pseudomonas aeruginosa PAO1, PA0092 (tsi6)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 113022 - 113306 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_248782.1
GI 15595290
Affymetrix PA0092_at
Entrez 880664
GenBank AAG03482.1
INSDC AAG03482.1
NCBI Locus Tag PA0092
protein_id(GenBank) gb|AAG03482.1|AE004448_1|gnl|PseudoCAP|PA0092
UniParc UPI00000C4F2D
UniProtKB Acc Q9I740
UniRef100 UniRef100_W0YKX2
UniRef50 UniRef50_A9BU08
UniRef90 UniRef90_W0YKX2


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.22
Kyte-Doolittle Hydrophobicity Value -0.126
Molecular Weight (kDa) 10.7
Isoelectric Point (pI) 7.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ZUY PROTEIN BINDING 05/18/15 Structure of Tsi6 from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 1.952 X-RAY DIFFRACTION 98.9
4ZV0 PROTEIN BINDING 05/18/15 Structure of Tse6 in complex with Tsi6 Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 1.401 X-RAY DIFFRACTION 100.0
5JTK UNKNOWN FUNCTION 05/09/16 NMR structure of Uncharacterized protein from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 7 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000091 (318 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0092
Search term: tsi6
Search term: Tsi6

Human Homologs


Genetically distinct pathways guide effector export through the type VI secretion system.
Whitney JC, Beck CM, Goo YA, Russell AB, Harding BN, De Leon JA, Cunningham DA, Tran BQ, Low DA, Goodlett DR, Hayes CS, Mougous JD
Mol Microbiol 2014 May;92(3):529-42
PubMed ID: 24589350
An interbacterial NAD(P)(+) glycohydrolase toxin requires elongation factor Tu for delivery to target cells.
Whitney JC, Quentin D, Sawai S, LeRoux M, Harding BN, Ledvina HE, Tran BQ, Robinson H, Goo YA, Goodlett DR, Raunser S, Mougous JD
Cell 2015 Oct 22;163(3):607-19
PubMed ID: 26456113