Pseudomonas aeruginosa PAO1, PA0143 (nuh)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0143
Name
nuh
Replicon chromosome
Genomic location 163363 - 164415 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentSpecific function outlined in reference. Nonspecific ribonucleoside hydrolase annotation ID (with class I confidence) implies that there are more reactions catalyzed by this enzyme.

Cross-References

RefSeq NP_248833.2
GI 119167454
Affymetrix PA0143_at
DNASU PaCD00006062
Entrez 879451
GenBank AAG03533.2
INSDC AAG03533.2
NCBI Locus Tag PA0143
protein_id(GenBank) gb|AAG03533.2|AE004452_5|gnl|PseudoCAP|PA0143
TIGR NTL03PA00144
UniParc UPI00000077AE
UniProtKB Acc Q9I6Y9
UniProtKB ID Q9I6Y9_PSEAE
UniRef100 UniRef100_Q9I6Y9
UniRef50 UniRef50_Q9I6Y9
UniRef90 UniRef90_Q9I6Y9

Product

Feature Type CDS
Coding Frame 1
Product Name
purine nucleosidase Nuh
Product Name Confidence: Class 1
Synonyms nucleoside hydrolase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.38
Kyte-Doolittle Hydrophobicity Value 0.029
Molecular Weight (kDa) 37.5
Isoelectric Point (pI) 7.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 241 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000141 (486 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0143
Search term: nuh

Human Homologs

References

Impact of quorum sensing on fitness of Pseudomonas aeruginosa.
Heurlier K, Dénervaud V, Haas D
Int. J. Med. Microbiol. 2006 Apr;296(2-3):93-102
PubMed ID: 16503417
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994