Pseudomonas aeruginosa PAO1, PA0143 (nuh)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0143
|
| Name |
nuh
|
| Replicon | chromosome |
| Genomic location | 163363 - 164415 (+ strand) |
| Transposon Mutants | 3 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
| Comment | Specific function outlined in reference. Nonspecific ribonucleoside hydrolase annotation ID (with class I confidence) implies that there are more reactions catalyzed by this enzyme. |
Cross-References
| RefSeq | NP_248833.2 |
| GI | 119167454 |
| Affymetrix | PA0143_at |
| DNASU | PaCD00006062 |
| Entrez | 879451 |
| GenBank | AAG03533.2 |
| INSDC | AAG03533.2 |
| NCBI Locus Tag | PA0143 |
| protein_id(GenBank) | gb|AAG03533.2|AE004452_5|gnl|PseudoCAP|PA0143 |
| TIGR | NTL03PA00144 |
| UniParc | UPI00000077AE |
| UniProtKB Acc | Q9I6Y9 |
| UniProtKB ID | Q9I6Y9_PSEAE |
| UniRef100 | UniRef100_Q9I6Y9 |
| UniRef50 | UniRef50_Q9I6Y9 |
| UniRef90 | UniRef90_Q9I6Y9 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
purine nucleosidase Nuh
|
| Synonyms |
nucleoside hydrolase |
| Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 1.38 |
| Kyte-Doolittle Hydrophobicity Value | 0.029 |
| Molecular Weight (kDa) | 37.5 |
| Isoelectric Point (pI) | 7.66 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 241 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000141 (486 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0143
Search term: nuh
Search term: purine nucleosidase Nuh
|
Human Homologs
References
|
Impact of quorum sensing on fitness of Pseudomonas aeruginosa.
Heurlier K, Dénervaud V, Haas D
Int. J. Med. Microbiol. 2006 Apr;296(2-3):93-102
PubMed ID: 16503417
|
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|