Pseudomonas aeruginosa PAO1, PA0154 (pcaG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0154
Name
pcaG
Replicon chromosome
Genomic location 175503 - 176108 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248844.1
GI 15595352
Affymetrix PA0154_pcaG_at
DNASU PaCD00006066
Entrez 882145
GenBank AAG03544.1
INSDC AAG03544.1
NCBI Locus Tag PA0154
protein_id(GenBank) gb|AAG03544.1|AE004453_7|gnl|PseudoCAP|PA0154
TIGR NTL03PA00155
UniParc UPI00000C4F69
UniProtKB Acc Q9I6X8
UniProtKB ID Q9I6X8_PSEAE
UniRef100 UniRef100_Q9I6X8
UniRef50 UniRef50_A6UXU3
UniRef90 UniRef90_A6UXU3

Product

Feature Type CDS
Coding Frame 1
Product Name
protocatechuate 3,4-dioxygenase, alpha subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) -6.14
Kyte-Doolittle Hydrophobicity Value -0.430
Molecular Weight (kDa) 22.8
Isoelectric Point (pI) 5.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 101 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000152 (447 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0154
Search term: pcaG

Human Homologs

References

Crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase: endogenous Fe3+ ligand displacement in response to substrate binding.
Orville AM, Lipscomb JD, Ohlendorf DH
Biochemistry 1997 Aug 19;36(33):10052-66
PubMed ID: 9254600