Pseudomonas aeruginosa PAO1, PA0169 (siaD)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0169
|
| Name |
siaD
|
| Replicon | chromosome |
| Genomic location | 192965 - 193672 (- strand) |
| Transposon Mutants | 1 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 6 transposon mutants in orthologs |
| Comment | Involved in surfactant-induced (SDS) aggregation. |
| Comment | Involved in postranscriptional regulation of CupA fimbriae via transcriptional activation of rsmZ |
Cross-References
| RefSeq | NP_248859.1 |
| GI | 15595367 |
| Affymetrix | PA0169_at |
| Entrez | 879346 |
| GenBank | AAG03559.1 |
| INSDC | AAG03559.1 |
| NCBI Locus Tag | PA0169 |
| protein_id(GenBank) | gb|AAG03559.1|AE004454_11|gnl|PseudoCAP|PA0169 |
| TIGR | NTL03PA00170 |
| UniParc | UPI00000C4F78 |
| UniProtKB Acc | Q9I6W3 |
| UniProtKB ID | Q9I6W3_PSEAE |
| UniRef100 | UniRef100_Q9I6W3 |
| UniRef50 | UniRef50_Q9I6W3 |
| UniRef90 | UniRef90_Q9I6W3 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
SiaD
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 4.91 |
| Kyte-Doolittle Hydrophobicity Value | -0.539 |
| Molecular Weight (kDa) | 26.8 |
| Isoelectric Point (pI) | 9.49 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 7E6G | DE NOVO PROTEIN | 02/22/21 | Crystal structure of diguanylate cyclase SiaD in complex with its activator SiaC from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.65 | X-RAY DIFFRACTION | 95.7 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 454 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000167 (2822 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0169
Search term: siaD
Search term: SiaD
|
Human Homologs
References
|
Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy.
Klebensberger J, Lautenschlager K, Bressler D, Wingender J, Philipp B
Environ. Microbiol. 2007 Sep;9(9):2247-59
PubMed ID: 17686022
|
|
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Klebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B
Environ. Microbiol. 2009 Dec;11(12):3073-86
PubMed ID: 19638175
|
|
SiaA/D Interconnects c-di-GMP and RsmA Signaling to Coordinate Cellular Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions.
Colley B, Dederer V, Carnell M, Kjelleberg S, Rice SA, Klebensberger J
Front Microbiol 2016 ;7
PubMed ID: 26955366
|