Pseudomonas aeruginosa PAO1, PA0176

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: mcpB
Replicon chromosome
Genomic location 199600 - 201639 (- strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPart of cluster II che genes involved in chemotaxis


RefSeq NP_248866.1
GI 15595374
Affymetrix PA0176_at
Entrez 882219
GenBank AAG03566.1
INSDC AAG03566.1
NCBI Locus Tag PA0176
protein_id(GenBank) gb|AAG03566.1|AE004455_7|gnl|PseudoCAP|PA0176
UniParc UPI00000C4F7E
UniProtKB Acc Q9I6V6
UniRef100 UniRef100_Q9I6V6
UniRef50 UniRef50_Q9I6V6
UniRef90 UniRef90_Q9I6V6


Feature Type CDS
Coding Frame 1
Product Name
aerotaxis transducer Aer2
Product Name Confidence: Class 1
Synonyms methyl-accepting chemotaxis protein, McpB
methyl-accepting chemotaxis protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -18.01
Kyte-Doolittle Hydrophobicity Value -0.257
Molecular Weight (kDa) 72.6
Isoelectric Point (pI) 4.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4HI4 SIGNALING PROTEIN 10/11/12 Crystal structure of the 5-coordinate ferric heme-binding PAS domain of Aer2 from P. aeruginosa Pseudomonas aeruginosa 2.304 X-RAY DIFFRACTION 95.8
3LNR SIGNALING PROTEIN 02/02/10 Crystal structure of poly-HAMP domains from the P. aeruginosa soluble receptor Aer2 Pseudomonas aeruginosa 2.64 X-RAY DIFFRACTION 100.0
3VOL SIGNALING PROTEIN 01/27/12 X-ray Crystal Structure of PAS-HAMP Aer2 in the CN-bound Form Pseudomonas aeruginosa 2.399 X-RAY DIFFRACTION 98.6
4I44 SIGNALING PROTEIN 11/27/12 Aer2 poly-HAMP domains: V33G HAMP1 inverted signaling mutant Pseudomonas aeruginosa 2.88 X-RAY DIFFRACTION 99.4
4I3M SIGNALING PROTEIN 11/26/12 Aer2 poly-HAMP domains: L44H HAMP1 CW-lock mutant Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 99.4

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 398 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000174 (385 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0176

Human Homologs


The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa.
Hong CS, Kuroda A, Ikeda T, Takiguchi N, Ohtake H, Kato J
J Biosci Bioeng 2004;97(3):184-90
PubMed ID: 16233612
Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa.
Hong CS, Shitashiro M, Kuroda A, Ikeda T, Takiguchi N, Ohtake H, Kato J
FEMS Microbiol Lett 2004 Feb 16;231(2):247-52
PubMed ID: 14987771
Sites of deamidation and methylation in Tsr, a bacterial chemotaxis sensory transducer.
Rice MS, Dahlquist FW
J Biol Chem 1991 May 25;266(15):9746-53
PubMed ID: 2033064
The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence.
García-Fontana C, Vílchez JI, González-Requena M, González-López J, Krell T, Matilla MA, Manzanera M
Sci Rep 2019 Sep 11;9(1):13166
PubMed ID: 31511598
Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response.
Ferrández A, Hawkins AC, Summerfield DT, Harwood CS
J Bacteriol 2002 Aug;184(16):4374-83
PubMed ID: 12142407
Two different Pseudomonas aeruginosa chemosensory signal transduction complexes localize to cell poles and form and remould in stationary phase.
Güvener ZT, Tifrea DF, Harwood CS
Mol Microbiol 2006 Jul;61(1):106-18
PubMed ID: 16824098