Pseudomonas aeruginosa PAO1, PA0183 (atsA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0183
Name
atsA
Replicon chromosome
Genomic location 207923 - 209533 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248873.1
GI 15595381
Affymetrix PA0183_atsA_at
Entrez 879288
GenBank AAG03573.1
INSDC AAG03573.1
NCBI Locus Tag PA0183
protein_id(GenBank) gb|AAG03573.1|AE004456_4|gnl|PseudoCAP|PA0183
TIGR NTL03PA00184
UniParc UPI0000165EBE
UniProtKB Acc P51691
UniProtKB ID ARS_PSEAE
UniRef100 UniRef100_P51691
UniRef50 UniRef50_P51691
UniRef90 UniRef90_P51691

Product

Feature Type CDS
Coding Frame 1
Product Name
arylsulfatase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -12.39
Kyte-Doolittle Hydrophobicity Value -0.378
Molecular Weight (kDa) 59.9
Isoelectric Point (pI) 5.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4CXS HYDROLASE 04/08/14 G4 mutant of PAS, arylsulfatase from Pseudomonas aeruginosa, in complex with Phenylphosphonic acid PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 98.7
5AJ9 HYDROLASE 02/20/15 G7 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 2 X-RAY DIFFRACTION 98.5
4CXU HYDROLASE 04/08/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with 3-Br-Phenolphenylphosphonate PSEUDOMONAS AERUGINOSA 2.03 X-RAY DIFFRACTION 98.7
4CYR HYDROLASE 04/14/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 1.72 X-RAY DIFFRACTION 98.7
4CXK HYDROLASE 04/07/14 G9 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 1.86 X-RAY DIFFRACTION 98.1
1HDH HYDROLASE 11/16/00 Arylsulfatase from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.3 X-RAY DIFFRACTION 99.8
4CYS HYDROLASE 04/14/14 G6 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid PSEUDOMONAS AERUGINOSA 1.88 X-RAY DIFFRACTION 98.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
2-Aminopropanedioic Acid DB02289 DrugBank 5.1.4 P51691 Arylsulfatase 100.0 536 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000181 (374 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0183
Search term: atsA
Search term: arylsulfatase

Human Homologs

Ensembl 107, assembly GRCh38.p13
arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714]
E-value: 3.3e-30
Percent Identity: 29.9
Ensembl 107, assembly GRCh38.p13
arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714]
E-value: 3.3e-30
Percent Identity: 29.9
Ensembl 107, assembly GRCh38.p13
arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714]
E-value: 3.3e-30
Percent Identity: 29.9

References

Purification and characterization of the arylsulfatase synthesized by Pseudomonas aeruginosa PAO during growth in sulfate-free medium and cloning of the arylsulfatase gene (atsA).
Beil S, Kehrli H, James P, Staudenmann W, Cook AM, Leisinger T, Kertesz MA
Eur. J. Biochem. 1995 Apr;229(2):385-94
PubMed ID: 7744061