Pseudomonas aeruginosa PAO1, PA0211 (mdcD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0211
Name
mdcD
Replicon chromosome
Genomic location 240071 - 240934 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248902.1
GI 15595408
Affymetrix PA0211_mdcD_at
Entrez 879489
GenBank AAG03600.1
INSDC AAG03600.1
NCBI Locus Tag PA0211
protein_id(GenBank) gb|AAG03600.1|AE004459_4|gnl|PseudoCAP|PA0211
TIGR NTL03PA00212
UniParc UPI00000C4F9A
UniProtKB Acc Q9I6S7
UniProtKB ID Q9I6S7_PSEAE
UniRef100 UniRef100_Q9I6S7
UniRef50 UniRef50_A0A085GVQ3
UniRef90 UniRef90_A6UY06

Product

Feature Type CDS
Coding Frame 1
Product Name
malonate decarboxylase beta subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.08
Kyte-Doolittle Hydrophobicity Value 0.041
Molecular Weight (kDa) 30.3
Isoelectric Point (pI) 4.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5VIT TRANSFERASE 04/17/17 Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with malonate Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) 2.203 X-RAY DIFFRACTION 100.0
5VJ1 TRANSFERASE 04/18/17 Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with coenzyme A Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) 2.995 X-RAY DIFFRACTION 100.0
5VIP TRANSFERASE 04/17/17 Crystal structure of Pseudomonas malonate decarboxylase MdcD-MdcE hetero-dimer Pseudomonas aeruginosa 1.857 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 556 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000210 (492 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0211
Search term: mdcD

Human Homologs

References

Sequence of a gene cluster from Klebsiella pneumoniae encoding malonate decarboxylase and expression of the enzyme in Escherichia coli.
Hoenke S, Schmid M, Dimroth P
Eur. J. Biochem. 1997 Jun;246(2):530-8
PubMed ID: 9208947