Pseudomonas aeruginosa PAO1, PA0288 (gpuA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 323616 - 324572 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentKnockout of gpuA or gpuP abolishes 3-guanidinopropionate utilization.


RefSeq NP_248979.1
GI 15595485
Affymetrix PA0288_speB1_at
Entrez 880781
GenBank AAG03677.1
INSDC AAG03677.1
NCBI Locus Tag PA0288
protein_id(GenBank) gb|AAG03677.1|AE004466_11|gnl|PseudoCAP|PA0288
UniParc UPI00000C4FE4
UniProtKB Acc Q9I6K2
UniRef100 UniRef100_Q9I6K2
UniRef50 UniRef50_Q9I6K2
UniRef90 UniRef90_Q9I6K2


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Synonyms proclavaminic acid amidino hydrolase, PAH
agmatine ureohydrolase
proclavaminate amidino hydrolase
Evidence for Translation
Charge (pH 7) -9.65
Kyte-Doolittle Hydrophobicity Value -0.013
Molecular Weight (kDa) 34.2
Isoelectric Point (pI) 5.18

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3NIQ HYDROLASE 06/16/10 Crystal structure of Pseudomonas aeruginosa guanidinopropionase Pseudomonas aeruginosa 2.07 X-RAY DIFFRACTION 100.0
3NIP HYDROLASE 06/16/10 Crystal structure of Pseudomonas aeruginosa guanidinopropionase complexed with 1,6-diaminohexane Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 437 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000285 (312 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0288
Search term: gpuA

Human Homologs

Ensembl 110, assembly GRCh38.p14
agmatinase [Source:HGNC Symbol;Acc:HGNC:18407]
E-value: 3.4e-78
Percent Identity: 46.9


Purification and properties of agmatine ureohydrolyase, a putrescine biosynthetic enzyme in Escherichia coli.
Satishchandran C, Boyle SM
J Bacteriol 1986 Mar;165(3):843-8
PubMed ID: 3081491
Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis.
Aidoo KA, Wong A, Alexander DC, Rittammer RA, Jensen SE
Gene 1994 Sep 15;147(1):41-6
PubMed ID: 8088547
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA
J Bacteriol 1995 Jul;177(13):3714-20
PubMed ID: 7601835
Molecular characterization and regulation of the aguBA operon, responsible for agmatine utilization in Pseudomonas aeruginosa PAO1.
Nakada Y, Jiang Y, Nishijyo T, Itoh Y, Lu CD
J Bacteriol 2001 Nov;183(22):6517-24
PubMed ID: 11673419