Pseudomonas aeruginosa PAO1, PA0288 (gpuA)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0288
|
| Name |
gpuA
|
| Replicon | chromosome |
| Genomic location | 323616 - 324572 (+ strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
| Comment | Knockout of gpuA or gpuP abolishes 3-guanidinopropionate utilization. |
Cross-References
| RefSeq | NP_248979.1 |
| GI | 15595485 |
| Affymetrix | PA0288_speB1_at |
| Entrez | 880781 |
| GenBank | AAG03677.1 |
| INSDC | AAG03677.1 |
| NCBI Locus Tag | PA0288 |
| protein_id(GenBank) | gb|AAG03677.1|AE004466_11|gnl|PseudoCAP|PA0288 |
| TIGR | NTL03PA00289 |
| UniParc | UPI00000C4FE4 |
| UniProtKB Acc | Q9I6K2 |
| UniProtKB ID | GPUA_PSEAE |
| UniRef100 | UniRef100_Q9I6K2 |
| UniRef50 | UniRef50_Q9I6K2 |
| UniRef90 | UniRef90_Q9I6K2 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
3-guanidinopropionase
|
| Synonyms |
proclavaminic acid amidino hydrolase, PAH agmatine ureohydrolase proclavaminate amidino hydrolase AUH |
| Evidence for Translation | |
| Charge (pH 7) | -9.65 |
| Kyte-Doolittle Hydrophobicity Value | -0.013 |
| Molecular Weight (kDa) | 34.2 |
| Isoelectric Point (pI) | 5.18 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 3NIP | HYDROLASE | 06/16/10 | Crystal structure of Pseudomonas aeruginosa guanidinopropionase complexed with 1,6-diaminohexane | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 100.0 |
| 3NIQ | HYDROLASE | 06/16/10 | Crystal structure of Pseudomonas aeruginosa guanidinopropionase | Pseudomonas aeruginosa | 2.07 | X-RAY DIFFRACTION | 100.0 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 437 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000285 (315 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0288
Search term: gpuA
Search term: 3-guanidinopropionase
|
Human Homologs
|
Ensembl
107, assembly
GRCh38.p13
|
agmatinase [Source:HGNC Symbol;Acc:HGNC:18407]
E-value:
3.3e-78
Percent Identity:
46.9
|
References
|
Purification and properties of agmatine ureohydrolyase, a putrescine biosynthetic enzyme in Escherichia coli.
Satishchandran C, Boyle SM
J Bacteriol 1986 Mar;165(3):843-8
PubMed ID: 3081491
|
|
Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis.
Aidoo KA, Wong A, Alexander DC, Rittammer RA, Jensen SE
Gene 1994 Sep;147(1):41-6
PubMed ID: 8088547
|
|
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA
J. Bacteriol. 1995 Jul;177(13):3714-20
PubMed ID: 7601835
|
|
Molecular characterization and regulation of the aguBA operon, responsible for agmatine utilization in Pseudomonas aeruginosa PAO1.
Nakada Y, Jiang Y, Nishijyo T, Itoh Y, Lu CD
J. Bacteriol. 2001 Nov;183(22):6517-24
PubMed ID: 11673419
|