Pseudomonas aeruginosa PAO1, PA0291 (oprE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 327284 - 328666 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).


RefSeq NP_248982.1
GI 15595488
Affymetrix PA0291_oprE_at
Entrez 879473
GenBank AAG03680.1
INSDC AAG03680.1
NCBI Locus Tag PA0291
protein_id(GenBank) gb|AAG03680.1|AE004467_2|gnl|PseudoCAP|PA0291
UniParc UPI00000D428C
UniProtKB Acc G3XDA5
UniRef100 UniRef100_G3XDA5
UniRef50 UniRef50_G3XDA5
UniRef90 UniRef90_G3XDA5


Feature Type CDS
Coding Frame 1
Product Name
Anaerobically-induced outer membrane porin OprE precursor
Product Name Confidence: Class 1
Synonyms OccK8
Porin E1
Evidence for Translation
Detected using LC-MS/MS (49.67 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 4.20
Kyte-Doolittle Hydrophobicity Value -0.436
Molecular Weight (kDa) 49.7
Isoelectric Point (pI) 9.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4FRX TRANSPORT PROTEIN 06/26/12 Crystal Structure of Pseudomonas aeruginosa OccK8 (OprE) Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 19 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000288 (506 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0291
Search term: oprE

Human Homologs


Identification of porins in the outer membrane of Pseudomonas aeruginosa that form small diffusion pores.
Yoshihara E, Nakae T
J Biol Chem 1989 Apr 15;264(11):6297-301
PubMed ID: 2539376
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
Responses of carbapenemase-producing and non-producing carbapenem-resistant Pseudomonas aeruginosa strains to meropenem revealed by quantitative tandem mass spectrometry proteomics.
Salvà-Serra F, Jaén-Luchoro D, Marathe NP, Adlerberth I, Moore ERB, Karlsson R
Front Microbiol 2022;13:1089140
PubMed ID: 36845973
Role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa.
Tamber S, Ochs MM, Hancock RE
J Bacteriol 2006 Jan;188(1):45-54
PubMed ID: 16352820