Pseudomonas aeruginosa PAO1, PA0297 (spuA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0297
Name
spuA
Synonym: ycjL
Replicon chromosome
Genomic location 334734 - 335486 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248988.1
GI 15595494
Affymetrix PA0297_at
DNASU PaCD00006130
Entrez 880604
GenBank AAG03686.1
INSDC AAG03686.1
NCBI Locus Tag PA0297
protein_id(GenBank) gb|AAG03686.1|AE004467_8|gnl|PseudoCAP|PA0297
TIGR NTL03PA00298
UniParc UPI00000C4FEC
UniProtKB Acc Q9I6J4
UniProtKB ID Q9I6J4_PSEAE
UniRef100 UniRef100_W0YL61
UniRef50 UniRef50_Q48IV7
UniRef90 UniRef90_W0YL61

Product

Feature Type CDS
Coding Frame 1
Product Name
probable glutamine amidotransferase
Product Name Confidence: Class 2
Synonyms pauD1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.45
Kyte-Doolittle Hydrophobicity Value -0.001
Molecular Weight (kDa) 26.5
Isoelectric Point (pI) 6.43

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7D4R HYDROLASE 09/24/20 SpuA native structure Pseudomonas aeruginosa PAO1 1.6 X-RAY DIFFRACTION 100.0
7D53 HYDROLASE 09/24/20 SpuA mutant - H221N with Glu Pseudomonas aeruginosa PAO1 1.6 X-RAY DIFFRACTION 99.6
7D50 HYDROLASE 09/24/20 SpuA mutant - H221N with glutamyl-thioester Pseudomonas aeruginosa PAO1 1.77 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 316 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000294 (491 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0297
Search term: spuA

Human Homologs

References

Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.
Yao X, He W, Lu CD
J. Bacteriol. 2011 Aug;193(15):3923-30
PubMed ID: 21622750