Pseudomonas aeruginosa PAO1, PA0301 (spuE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0301
Name
spuE
Synonym: potF3
Replicon chromosome
Genomic location 339959 - 341056 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248992.1
GI 15595498
Affymetrix PA0301_potF3_at
Entrez 877626
GenBank AAG03690.1
INSDC AAG03690.1
NCBI Locus Tag PA0301
protein_id(GenBank) gb|AAG03690.1|AE004468_2|gnl|PseudoCAP|PA0301
TIGR NTL03PA00302
UniParc UPI00000C4FF0
UniProtKB Acc Q9I6J0
UniProtKB ID SPUE_PSEAE
UniRef100 UniRef100_Q9I6J0
UniRef50 UniRef50_Q9I6J0
UniRef90 UniRef90_Q9I6J0

Product

Feature Type CDS
Coding Frame 1
Product Name
polyamine transport protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.86
Kyte-Doolittle Hydrophobicity Value -0.193
Molecular Weight (kDa) 40.1
Isoelectric Point (pI) 5.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6IKM ANTIMICROBIAL PROTEIN 10/16/18 Crystal structure of SpuE-Spermidine in complex with ScFv5 Homo sapiens; Pseudomonas aeruginosa 3.398 X-RAY DIFFRACTION 100.0
3TTL TRANSPORT PROTEIN 09/14/11 Crystal structure of apo-SpuE Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 100.0
3TTN TRANSPORT PROTEIN 09/15/11 Crystal structures of polyamine receptors SpuD and SpuE from Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 308 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000298 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0301
Search term: spuE

Human Homologs

References

Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome.
Pistocchi R, Kashiwagi K, Miyamoto S, Nukui E, Sadakata Y, Kobayashi H, Igarashi K
J Biol Chem 1993 Jan 5;268(1):146-52
PubMed ID: 8416922
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299