Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0301
|
Name |
spuE
Synonym: potF3 |
Replicon | chromosome |
Genomic location | 339959 - 341056 (+ strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_248992.1 |
GI | 15595498 |
Affymetrix | PA0301_potF3_at |
Entrez | 877626 |
GenBank | AAG03690.1 |
INSDC | AAG03690.1 |
NCBI Locus Tag | PA0301 |
protein_id(GenBank) | gb|AAG03690.1|AE004468_2|gnl|PseudoCAP|PA0301 |
TIGR | NTL03PA00302 |
UniParc | UPI00000C4FF0 |
UniProtKB Acc | Q9I6J0 |
UniProtKB ID | SPUE_PSEAE |
UniRef100 | UniRef100_Q9I6J0 |
UniRef50 | UniRef50_Q9I6J0 |
UniRef90 | UniRef90_Q9I6J0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
polyamine transport protein
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -4.86 |
Kyte-Doolittle Hydrophobicity Value | -0.193 |
Molecular Weight (kDa) | 40.1 |
Isoelectric Point (pI) | 5.47 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3TTL | TRANSPORT PROTEIN | 09/14/11 | Crystal structure of apo-SpuE | Pseudomonas aeruginosa | 2.3 | X-RAY DIFFRACTION | 100.0 |
3TTN | TRANSPORT PROTEIN | 09/15/11 | Crystal structures of polyamine receptors SpuD and SpuE from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
6IKM | ANTIMICROBIAL PROTEIN | 10/16/18 | Crystal structure of SpuE-Spermidine in complex with ScFv5 | Homo sapiens; Pseudomonas aeruginosa | 3.398 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 308 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000298 (533 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0301
Search term: spuE
Search term: polyamine transport protein
|
Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
|
Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome.
Pistocchi R, Kashiwagi K, Miyamoto S, Nukui E, Sadakata Y, Kobayashi H, Igarashi K
J Biol Chem 1993 Jan 5;268(1):146-52
PubMed ID: 8416922
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299
|