Pseudomonas aeruginosa PAO1, PA0363 (coaD)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: kdtB
Replicon chromosome
Genomic location 406619 - 407098 (- strand)


RefSeq NP_249054.1
GI 15595560
Affymetrix PA0363_coaD_at
Entrez 879847
GenBank AAG03752.1
INSDC AAG03752.1
NCBI Locus Tag PA0363
protein_id(GenBank) gb|AAG03752.1|AE004474_4|gnl|PseudoCAP|PA0363
UniParc UPI0000127CC2
UniProtKB Acc Q9I6D1
UniRef100 UniRef100_B7V2S6
UniRef50 UniRef50_Q5ZY26
UniRef90 UniRef90_B7V2S6


Feature Type CDS
Coding Frame 1
Product Name
phosphopantetheine adenylyltransferase
Product Name Confidence: Class 2
Synonyms PPAT
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.19
Kyte-Doolittle Hydrophobicity Value -0.007
Molecular Weight (kDa) 17.8
Isoelectric Point (pI) 8.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3X1M TRANSFERASE 11/24/14 Crystal structure of Phosphopantetheine adenylyltransferase/PPAT from Pseudomonas aeruginosa with CoA PSEUDOMONAS AERUGINOSA 2192 2.5 X-RAY DIFFRACTION 100.0
5TS2 TRANSFERASE 10/27/16 Crystal structure of a phosphopantetheine adenylyltransferase (CoaD, PPAT) from Pseudomonas aeruginosa bound to dephospho coenzyme A PSEUDOMONAS AERUGINOSA (STRAIN LESB58) 2.3 X-RAY DIFFRACTION 100.0
4RUK TRANSFERASE 11/20/14 crystal structure of Phosphoapantetheine adenylyltransferase PPAT/CoaD with CoA and pyrophosphate from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2192 2.2 X-RAY DIFFRACTION 100.0
3X1K TRANSFERASE 11/20/14 crystal structure of Phosphoapantetheine adenylyltransferase PPAT/CoaD with AMP-PNP from Pseudomonas aerugonosa PSEUDOMONAS AERUGINOSA 2192 2.547 X-RAY DIFFRACTION 100.0
3X1J TRANSFERASE 11/19/14 Crystal Structure of Phosphopantetheine adenylyltransferase (PPAT/CoaD) with AcCoA from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2192 2.334 X-RAY DIFFRACTION 100.0
5X6F TRANSFERASE 02/21/17 Crystal structure of Phosphopantetheine adenylyltransferase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.593 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 578 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000354 (554 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0363
Search term: coaD


A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing.
Clementz T, Raetz CR
J Biol Chem 1991 May 25;266(15):9687-96
PubMed ID: 2033061
Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus.
Roncero C, Casadaban MJ
J Bacteriol 1992 May;174(10):3250-60
PubMed ID: 1577693
Purification and characterization of phosphopantetheine adenylyltransferase from Escherichia coli.
Geerlof A, Lewendon A, Shaw WV
J. Biol. Chem. 1999 Sep;274(38):27105-11
PubMed ID: 10480925