Pseudomonas aeruginosa PAO1, PA0622

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 681394 - 682554 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).


RefSeq NP_249313.1
GI 15595819
Affymetrix PA0622_at
DNASU PaCD00006275
Entrez 877994
GenBank AAG04011.1
INSDC AAG04011.1
NCBI Locus Tag PA0622
protein_id(GenBank) gb|AAG04011.1|AE004498_6|gnl|PseudoCAP|PA0622
UniParc UPI00000D4266
UniProtKB Acc G3XD39
UniRef100 UniRef100_G3XD39
UniRef50 UniRef50_Q02TE1
UniRef90 UniRef90_Q02TE1


Feature Type CDS
Coding Frame 1
Product Name
probable bacteriophage protein
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (41.21 kDa, PMID:21751344)
Charge (pH 7) -5.36
Kyte-Doolittle Hydrophobicity Value -0.012
Molecular Weight (kDa) 41.2
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3J9R STRUCTURAL PROTEIN 02/17/15 Atomic structures of a bactericidal contractile nanotube in its pre- and post-contraction states PSEUDOMONAS AERUGINOSA 3.9 ELECTRON MICROSCOPY 100.0
3J9Q STRUCTURAL PROTEIN 02/17/15 Atomic structures of a bactericidal contractile nanotube in its pre- and post-contraction states PSEUDOMONAS AERUGINOSA 3.5 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 152 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000605 (333 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0622


Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878