Pseudomonas aeruginosa PAO1, PA0895 (aruC)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: argD
Synonym: dapC
Synonym: astC
Replicon chromosome
Genomic location 977910 - 979130 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentaruC is an aminotransferase gene that has dual function in arginine catabolism (as SOAT) and in arginine biosynthesis (as ACOAT)


RefSeq NP_249586.1
GI 15596092
Affymetrix PA0895_aruC_at
Entrez 878207
GenBank AAG04284.1
INSDC AAG04284.1
NCBI Locus Tag PA0895
protein_id(GenBank) gb|AAG04284.1|AE004524_1|gnl|PseudoCAP|PA0895
UniParc UPI00001260C7
UniProtKB Acc O30508
UniRef100 UniRef100_O30508
UniRef50 UniRef50_P40732
UniRef90 UniRef90_O30508


Feature Type CDS
Coding Frame 1
Product Name
N2-Succinylornithine 5-aminotransferase (SOAT) = N2-acetylornithine 5-aminotransferase (ACOAT)
Product Name Confidence: Class 1
Synonyms N-succinylglutamate 5-semialdehyde dehydrogenase
succinylornithine aminotransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.40
Kyte-Doolittle Hydrophobicity Value -0.087
Molecular Weight (kDa) 43.7
Isoelectric Point (pI) 5.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 639 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000862 (553 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0895
Search term: aruC

Human Homologs

Ensembl 97, assembly GRCh38.p12
ornithine aminotransferase [Source:HGNC Symbol;Acc:HGNC:8091]
E-value: 2.4e-61
Percent Identity: 34.6


Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y
J. Bacteriol. 1997 Dec;179(23):7280-90
PubMed ID: 9393691
Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli.
Schneider BL, Kiupakis AK, Reitzer LJ
J. Bacteriol. 1998 Aug;180(16):4278-86
PubMed ID: 9696779
The dual biosynthetic capability of N-acetylornithine aminotransferase in arginine and lysine biosynthesis.
Ledwidge R, Blanchard JS
Biochemistry 1999 Mar;38(10):3019-24
PubMed ID: 10074354
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A, Stalon V, Wauven CV, Leisinger T, Haas D
Proc. Natl. Acad. Sci. U.S.A. 1986 Jul;83(13):4937-41
PubMed ID: 16593724