Pseudomonas aeruginosa PAO1, PA0899 (aruB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0899
Name
aruB
Replicon chromosome
Genomic location 982885 - 984231 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249590.1
GI 15596096
Affymetrix PA0899_aruB_at
Entrez 878588
GenBank AAG04288.1
INSDC AAG04288.1
NCBI Locus Tag PA0899
protein_id(GenBank) gb|AAG04288.1|AE004524_5|gnl|PseudoCAP|PA0899
TIGR NTL03PA00900
UniParc UPI00001261AF
UniProtKB Acc O50175
UniProtKB ID ASTB_PSEAE
UniRef100 UniRef100_B7UY35
UniRef50 UniRef50_Q2SKP0
UniRef90 UniRef90_A6VA75

Product

Feature Type CDS
Coding Frame 1
Product Name
N2-Succinylarginine dihydrolase
Product Name Confidence: Class 1
Synonyms succinylarginine dihydrolase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.62
Kyte-Doolittle Hydrophobicity Value -0.248
Molecular Weight (kDa) 48.9
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 65 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000866 (508 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0899
Search term: aruB

Human Homologs

References

Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y
J Bacteriol 1997 Dec;179(23):7280-90
PubMed ID: 9393691
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A, Stalon V, Wauven CV, Leisinger T, Haas D
Proc Natl Acad Sci U S A 1986 Jul;83(13):4937-41
PubMed ID: 16593724