Pseudomonas aeruginosa PAO1, PA0907 (alpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0907
Name
alpA
Replicon chromosome
Genomic location 992714 - 993244 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
CommentActivator of the alp lysis genes

Cross-References

RefSeq NP_249598.1
GI 15596104
Affymetrix PA0907_at
DNASU PaCD00006418
Entrez 878349
GenBank AAG04296.1
INSDC AAG04296.1
NCBI Locus Tag PA0907
protein_id(GenBank) gb|AAG04296.1|AE004525_4|gnl|PseudoCAP|PA0907
TIGR NTL03PA00908
UniParc UPI00000C51AE
UniProtKB Acc Q9I550
UniProtKB ID Q9I550_PSEAE
UniRef100 UniRef100_W0YMZ6
UniRef50 UniRef50_W0YMZ6
UniRef90 UniRef90_W0YMZ6

Product

Feature Type CDS
Coding Frame 1
Product Name
lysis phenotype activator, AlpA
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.20
Kyte-Doolittle Hydrophobicity Value -0.290
Molecular Weight (kDa) 20.2
Isoelectric Point (pI) 10.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7XYA TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loading complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.3 ELECTRON MICROSCOPY 93.1
7XYB TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loaded complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.7 ELECTRON MICROSCOPY 93.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 3 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000873 (301 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0907
Search term: alpA

Human Homologs

References

A self-lysis pathway that enhances the virulence of a pathogenic bacterium.
McFarland KA, Dolben EL, LeRoux M, Kambara TK, Ramsey KM, Kirkpatrick RL, Mougous JD, Hogan DA, Dove SL
Proc Natl Acad Sci U S A 2015 Jul 7;112(27):8433-8
PubMed ID: 26100878