Pseudomonas aeruginosa PAO1, PA0936 (lpxO2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0936
Name
lpxO2
Replicon chromosome
Genomic location 1026274 - 1027212 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLpxO2 appears to be responsible for most of lipid A hydroxylation in Pseudomonas aeruginosa PAO1 (PMID:31835493).

Cross-References

RefSeq NP_249627.1
GI 15596133
Affymetrix PA0936_at
DNASU PaCD00006428
Entrez 878312
GenBank AAG04325.1
INSDC AAG04325.1
NCBI Locus Tag PA0936
protein_id(GenBank) gb|AAG04325.1|AE004528_3|gnl|PseudoCAP|PA0936
TIGR NTL03PA00937
UniParc UPI00000C51C8
UniProtKB Acc Q9I522
UniProtKB ID Q9I522_PSEAE
UniRef100 UniRef100_Q9I522
UniRef50 UniRef50_Q4KGI6
UniRef90 UniRef90_Q9I522

Product

Feature Type CDS
Coding Frame 1
Product Name
lipopolysaccharide biosynthetic protein LpxO2
Product Name Confidence: Class 1
Synonyms lipopolysaccharide biosynthetic protein Lpx02
Evidence for Translation
Detected using LC-MS/MS (35.76 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 16.34
Kyte-Doolittle Hydrophobicity Value -0.138
Molecular Weight (kDa) 35.8
Isoelectric Point (pI) 10.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 84 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000902 (489 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0936
Search term: lpxO2

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Oxygen requirement for the biosynthesis of the S-2-hydroxymyristate moiety in Salmonella typhimurium lipid A. Function of LpxO, A new Fe2+/alpha-ketoglutarate-dependent dioxygenase homologue.
Gibbons HS, Lin S, Cotter RJ, Raetz CR
J Biol Chem 2000 Oct 20;275(42):32940-9
PubMed ID: 10903325
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Genetic Basis and Physiological Effects of Lipid A Hydroxylation in Pseudomonas aeruginosa PAO1.
Lo Sciuto A, Cervoni M, Stefanelli R, Spinnato MC, Di Giamberardino A, Mancone C, Imperi F
Pathogens 2019 Dec 10;8(4)
PubMed ID: 31835493