Pseudomonas aeruginosa PAO1, PA0973 (oprL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0973
Name
oprL
Synonym: pal
Synonym: excC
Replicon chromosome
Genomic location 1057400 - 1057906 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249664.1
GI 15596170
Affymetrix PA0973_oprL_at
DNASU PaCD00006446
Entrez 882991
GenBank AAG04362.1
INSDC AAG04362.1
NCBI Locus Tag PA0973
protein_id(GenBank) gb|AAG04362.1|AE004530_15|gnl|PseudoCAP|PA0973
TIGR NTL03PA00974
UniParc UPI00000C51E3
UniProtKB Acc Q9I4Z4
UniProtKB ID PAL_PSEAE
UniRef100 UniRef100_Q9I4Z4
UniRef50 UniRef50_P0A138
UniRef90 UniRef90_P0A138

Product

Feature Type CDS
Coding Frame 1
Product Name
Peptidoglycan associated lipoprotein OprL precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (17.92 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using 2-DE, MALDI-TOF/MS analysis (17.93 kDa, pI 5.9, PMID:16335974)
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Charge (pH 7) -1.32
Kyte-Doolittle Hydrophobicity Value -0.432
Molecular Weight (kDa) 17.9
Isoelectric Point (pI) 6.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 411 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000938 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0973
Search term: oprL

Human Homologs

References

Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
Nucleotide sequence of the gene for the peptidoglycan-associated lipoprotein of Escherichia coli K12.
Chen R, Henning U
Eur J Biochem 1987 Feb 16;163(1):73-7
PubMed ID: 3545827
Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli.
Levengood SK, Webster RE
J Bacteriol 1989 Dec;171(12):6600-9
PubMed ID: 2687247
The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL).
Lazzaroni JC, Portalier R
Mol Microbiol 1992 Mar;6(6):735-42
PubMed ID: 1574003
Molecular and immunological characterization of OprL, the 18 kDa outer-membrane peptidoglycan-associated lipoprotein (PAL) of Pseudomonas aeruginosa.
Lim A, De Vos D, Brauns M, Mossialos D, Gaballa A, Qing D, Cornelis P
Microbiology (Reading, Engl.) 1997 May;143 ( Pt 5)
PubMed ID: 9168620
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344