Pseudomonas aeruginosa PAO1, PA1097 (fleQ)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA1097
|
| Name |
fleQ
|
| Replicon | chromosome |
| Genomic location | 1187587 - 1189059 (+ strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_249788.1 |
| GI | 15596294 |
| Affymetrix | PA1097_fleQ_at |
| Entrez | 881960 |
| GenBank | AAG04486.1 |
| INSDC | AAG04486.1 |
| NCBI Locus Tag | PA1097 |
| protein_id(GenBank) | gb|AAG04486.1|AE004540_6|gnl|PseudoCAP|PA1097 |
| TIGR | NTL03PA01098 |
| UniParc | UPI00000D421B |
| UniProtKB Acc | G3XCV0 |
| UniProtKB ID | G3XCV0_PSEAE |
| UniRef100 | UniRef100_G3XCV0 |
| UniRef50 | UniRef50_G3XCV0 |
| UniRef90 | UniRef90_G3XCV0 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
transcriptional regulator FleQ
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -13.91 |
| Kyte-Doolittle Hydrophobicity Value | -0.364 |
| Molecular Weight (kDa) | 55.3 |
| Isoelectric Point (pI) | 5.03 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4WXM | TRANSCRIPTION REGULATOR | 11/14/14 | FleQ REC domain from Pseudomonas aeruginosa PAO1 | Pseudomonas aeruginosa PAO1 | 2.3 | X-RAY DIFFRACTION | 100.0 |
| 5EXS | TRANSCRIPTION | 11/24/15 | AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to ATP-gamma-S | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 100.0 |
| 7EJW | TRANSCRIPTION | 04/02/21 | Crystal structure of FleN in complex with FleQ AAA+ doamain | Pseudomonas aeruginosa PAO1 | 1.98 | X-RAY DIFFRACTION | 99.6 |
| 5EXT | TRANSCRIPTION | 11/24/15 | AAA+ domain of FleQ from Pseudomonas aeruginosa bound to ADP | Pseudomonas aeruginosa | 2.4 | X-RAY DIFFRACTION | 100.0 |
| 5EXP | TRANSCRIPTION | 11/24/15 | AAA+ domain of FleQ from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
| 5EXX | TRANSCRIPTION | 11/24/15 | AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to c-di-GMP | Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 3.311 | X-RAY DIFFRACTION | 100.0 |
| 6JDL | TRANSCRIPTION | 02/01/19 | Central domain of FleQ H287A mutant in complex with ATPgS and Mg | Pseudomonas aeruginosa | 2.249 | X-RAY DIFFRACTION | 99.2 |
| 6J7E | TRANSCRIPTION | 01/18/19 | Crystal Structure of Central domain of FleQ in complex with ATPgS and Mg | Pseudomonas aeruginosa | 2.4 | X-RAY DIFFRACTION | 99.6 |
| 6JDI | TRANSCRIPTION | 02/01/19 | Central domain of FleQ H287N mutant in complex with ATPgS and Mg | Pseudomonas aeruginosa | 1.95 | X-RAY DIFFRACTION | 99.2 |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0273 |
| Category | Flagella |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | 14617143 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 453 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG001058 (532 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA1097
Search term: fleQ
Search term: transcriptional regulator FleQ
|
Human Homologs
References
|
FleQ, the major flagellar gene regulator in Pseudomonas aeruginosa, binds to enhancer sites located either upstream or atypically downstream of the RpoN binding site.
Jyot J, Dasgupta N, Ramphal R
J Bacteriol 2002 Oct;184(19):5251-60
PubMed ID: 12218010
|
|
A transcriptional activator, FleQ, regulates mucin adhesion and flagellar gene expression in Pseudomonas aeruginosa in a cascade manner.
Arora SK, Ritchings BW, Almira EC, Lory S, Ramphal R
J Bacteriol 1997 Sep;179(17):5574-81
PubMed ID: 9287015
|