Pseudomonas aeruginosa PAO1, PA1288 (odsT)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1288
Name	odsT Synonym: fadL Synonym: ompP1
Replicon	chromosome
Genomic location	1399231 - 1400505 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq	NP_249979.1
GI	15596485
Affymetrix	PA1288_at
Entrez	881473
GenBank	AAG04677.1
INSDC	AAG04677.1
NCBI Locus Tag	PA1288
protein_id(GenBank)	gb|AAG04677.1|AE004558_6|gnl|PseudoCAP|PA1288
TIGR	NTL03PA01289
UniParc	UPI00000C52F0
UniProtKB Acc	Q9I456
UniProtKB ID	Q9I456_PSEAE
UniRef100	UniRef100_A0A069Q437
UniRef50	UniRef50_A0A069Q437
UniRef90	UniRef90_A0A069Q437

Product

Feature Type	CDS
Coding Frame	1
Product Name	oxylipin transporter Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Detected using LC-MS/MS (45.56 kDa, PMID:21751344) Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-3.56
Kyte-Doolittle Hydrophobicity Value	-0.263
Molecular Weight (kDa)	45.6
Isoelectric Point (pI)	5.98

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Class 3 20472543 Outer Membrane Vesicle Class 1 Outer Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 203 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001233 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1288 Search term: odsT Search term: oxylipin transporter

Human Homologs

References

Nucleotide sequencing and expression of the fadL gene involved in long-chain fatty acid transport in Escherichia coli. Said B, Ghosn CR, Vu L, Nunn WD Mol Microbiol 1988 May;2(3):363-70 PubMed ID: 2840553

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix. Toyofuku M, Roschitzki B, Riedel K, Eberl L J Proteome Res 2012 Oct 5;11(10):4906-15 PubMed ID: 22909304

Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms. Couto N, Schooling SR, Dutcher JR, Barber J J Proteome Res 2015 Oct 2;14(10):4207-22 PubMed ID: 26303878

Pseudomonas aeruginosa Secretes the Oxylipin Autoinducer Synthases OdsA and OdsB via the Xcp Type 2 Secretion System. Martínez E, Orihuela CJ, Campos-Gomez J J Bacteriol 2022 Jul 19;204(7):e0011422 PubMed ID: 35658521